Toxoplasma gondii sequesters lysosomes from mammalian hosts in the vacuolar space
Toxoplasma gondii sequesters lysosomes from mammalian hosts in the vacuolar space
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DOI：
10.1016/j.cell.2006.01.056
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发表时间：
2006-04-21
期刊：
影响因子：
64.5
通讯作者：
Joiner, KA
中科院分区：
文献类型：
--
作者：
Coppens, I;Dunn, JD;Joiner, KA
弓形虫的细胞内区室满足了寄生虫在哺乳动物细胞中快速生长的营养需求。我们证明，T.刚地虫通过利用宿主内-溶酶体系统积累来自宿主哺乳动物细胞的物质。寄生虫积极招募宿主微管，导致内溶酶体选择性吸引PV。PV膜的基于微管的内陷充当用于在PV内递送宿主内溶体的导管。这些管状导管由寄生虫外套装饰，包括管发生蛋白GRA7，其作用像一个绞喉，将宿主内吞细胞器隔离在液泡空间中。这些数据定义了一个意想不到的过程，使寄生虫亲密和集中访问的各种范围内的低分子量组分产生的内溶酶体系统。更一般地说，他们确定了一个独特的机制，单向运输和封存的主机细胞器。
The intracellular compartment harboring Toxoplasma gondii satisfies the parasite's nutritional needs for rapid growth in mammalian cells. We demonstrate that the parasitophorous vacuole (PV) of T. gondii accumulates material coming from the host mammalian cell via the exploitation of the host endo-lysosomal system. The parasite actively recruits host microtubules, resulting in selective attraction of endo-lysosomes to the PV. Microtubule-based invaginations of the PV membrane serve as conduits for the delivery of host endo-lysosomes within the PV. These tubular conduits are decorated by a parasite coat, including the tubulogenic protein GRA7, which acts like a garrote that sequesters host endocytic organelles in the vacuolar space. These data define an unanticipated process allowing the parasite intimate and concentrated access to a diverse range of low molecular weight components produced by the endo-lysosomal system. More generally, they identify a unique mechanism for unidirectional transport and sequestration of host organelles.