Term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery
Term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery
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DOI：
10.1016/j.placenta.2014.10.007
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发表时间：
2014-12-01
期刊：
影响因子：
3.8
通讯作者：
Klein, N.
中科院分区：
文献类型：
--
作者：
Doyle, R. M.;Alber, D. G.;Klein, N.
感染被认为是早产的可能触发因素，有证据表明早产的胎盘和胎盘膜中存在细菌。在这项研究中，16S rDNA 焦磷酸测序用于鉴定胎盘膜中的细菌。剖腹产和足月阴道分娩被发现具有共同的属。人型支原体、克里斯滕森气球菌、阴道加德纳菌和具核梭杆菌要么仅存在于早产儿胎膜中，要么比足月时丰度更高。这些数据支持之前使用靶向 qPCR 或广泛 16S rDNA PCR 和克隆的研究，但不支持最近使用高通量测序对胎盘组织进行微生物组分析。 (C) 2014 年作者。由爱思唯尔有限公司出版
Infection is considered a possible trigger for preterm labour, supported by evidence showing the presence of bacteria in the placenta and placental membranes from preterm births. In this study, 16S rDNA pyrosequencing was used to identify bacteria in placental membranes. Caesarean sections and vaginal deliveries at term were found to harbour common genera. Mycoplasma hominis, Aerococcus christensenii, Gardnerella vaginalis and Fusobacterium nucleatum were either only present in preterm membranes or in greater abundance than at term. These data support previous studies that used either targeted qPCR or broad-range 16S rDNA PCR and cloning but not a recent microbiome analysis of placental tissue using high-throughput sequencing. (C) 2014 The Authors. Published by Elsevier Ltd.