The Use of Human Wharton's Jelly Cells for Cochlear Tissue Engineering.
The Use of Human Wharton's Jelly Cells for Cochlear Tissue Engineering.
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使用人类沃顿胶细胞进行耳蜗组织工程。
DOI：
10.1007/978-1-4939-3615-1_19
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Staecker,Hinrich
中科院分区：
文献类型：
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作者：
Mellott,AdamJ;Detamore,MichaelS;Staecker,Hinrich
组织工程关注三个主要组成部分：干细胞、生物材料和生长因子。总之，这些成分的组合被用来再生和修复通常不容易自己再生的受损组织。由于胚胎干细胞（ESCs）和诱导多能干细胞（iPSCs）具有广泛的分化潜力，其应用受到了广泛的关注。然而，ESCs和iPSCs需要非常详细的方案才能分化成目标组织，这并不总是成功的。此外，在某些地区，获取ESCs被认为在伦理上存在争议，而获取iPSCs需要对成人组织进行艰苦的转化和鉴定。然而，间充质干细胞是一种成体干细胞群体，在伦理上没有争议，而且很容易获得。此外，间充质干细胞表现出从中胚层分化成多种细胞类型的能力。特别是人类沃顿氏水母细胞（hWJCs）是在脐带中发现的间充质型干细胞，具有显著的分化潜力。hwjc是一种非常理想的干细胞群体，因为它们供应丰富，增殖率高，并且能够从所有三个胚层分化成多种细胞类型。hwjc被用来产生来自外胚层的几种神经表型，并被认为是听觉复合体中发现的机械感觉毛细胞的工程。在这里，我们报道了从人脐带分离hWJCs和非病毒转染用于耳蜗组织工程研究的方法。
Tissue engineering focuses on three primary components: stem cells, biomaterials, and growth factors. Together, the combination of these components is used to regrow and repair damaged tissues that normally do not regenerate easily on their own. Much attention has been focused on the use of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), due to their broad differentiation potential. However, ESCs and iPSCs require very detailed protocols to differentiate into target tissues, which are not always successful. Furthermore, procurement of ESCs is considered ethically controversial in some regions and procurement of iPSCs requires laborious transformation of adult tissues and characterization. However, mesenchymal stem cells are an adult stem cell population that are not ethically controversial and are readily available for procurement. Furthermore, mesenchymal stem cells exhibit the ability to differentiate into a variety of cell types arising from the mesoderm. In particular, human Wharton’s jelly cells (hWJCs) are mesenchymal-type stem cells found in umbilical cords that possess remarkable differentiation potential. hWJCs are a highly desirable stem cell population due to their abundance in supply, high proliferation rates, and ability to differentiate into multiple cell types arising from all three germ layers. hWJCs are used to generate several neurological phenotypes arising from the ectoderm and are considered for engineering mechanosensory hair cells found in the auditory complex. Here, we report the methods for isolating hWJCs from human umbilical cords and non-virally transfected for use in cochlear tissue engineering studies.