Bio-functionalized thermoresponsive interfaces facilitating cell adhesion and proliferation
Bio-functionalized thermoresponsive interfaces facilitating cell adhesion and proliferation
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DOI：
10.1016/j.biomaterials.2006.05.019
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发表时间：
2006-10-01
期刊：
影响因子：
14
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Hatakeyama, Hideyuki;Kikuchi, Akihiko;Okano, Teruo
研究了细胞黏附肽RGDS和细胞生长因子胰岛素(INS)共固定的生物功能化温敏培养界面，以促进细胞的初始黏附和细胞生长，为进一步的细胞片工程应用奠定了基础。这些生物功能化界面是通过电子束诱导N-异丙基丙烯酰胺(IPAAm)与其羧基衍生类似物2-羧基异丙基丙烯酰胺(CIPAAm)共聚，然后接枝到组织培养聚苯乙烯培养皿上，然后将RGDS和/或INS固定到CIPAAm羧基上而制备的。检测牛颈动脉内皮细胞在RGDS-INS共固定化温敏界面上的黏附和增殖情况。固定化RGDS促进了EC在表面的初始黏附，INS修饰分别促进了EC的增殖。通过共固定化适量的RGDS和INS，表明EC的生长更加明显。这可能是由于表面固定的RGDS和INS分子对贴壁内皮细胞的直接共刺激的协同作用。生长在RGDS-INS共固定化热响应性界面上的ECs也可以通过单独降低培养温度自发恢复为活性组织单层。RGDS-INS共固定的热响应界面比未修饰的热响应表面更能支持EC的初始黏附和生长，即使在无血清培养下也是如此。在无血清培养基中加入可溶性生长因子能有效地诱导EC增殖融合。将细胞黏附肽和生长因子共固定化在温度响应性表面，可有效地快速制备完整的细胞膜，并将其用于再生医学。(C)2006爱思唯尔有限公司。保留所有权利。
Bio-functionalized thermoresponsive culture interfaces co-immobilized with cell adhesive peptide, RGDS, and cell growth factor, insulin (INS), are investigated to promote initial cell adhesion and cell growth for further cell sheet engineering applications. These biofunctionalized interfaces were prepared by electron beam-induced copolymerization of N-isopropylacrylamide (IPAAm) with its carboxyl-derivatized analog, 2-carboxyisopropylacrylamide (CIPAAm), and grafting onto tissue culture polystyrene dishes, followed by immobilization of RGDS and/or INS to CIPAAm carboxyls. Adhesion and proliferation of bovine carotid artery endothelial cells (ECs) were examined on the RGDS-INS co-immobilized thermoresponsive interfaces. Immobilized RGDS facilitated initial EC adhesion on the surfaces and INS modification was demonstrated to induce EC proliferation, respectively. More pronounced EC growth was indicated by co-immobilization of appropriate amount of RGDS and INS. This may be due to synergistic effect of direct co-stimulation of adhered ECs by surface-immobilized RGDS and INS molecules. ECs grown on the RGDS-INS co-immobilized thermoresponsive interfaces can also be recovered spontaneously as viable tissue monolayers by solely reducing culture temperature. RGDS-INS co-immobilized thermoresponsive interfaces strongly supported initial EC adhesion and growth than unmodified thermoresponsive surfaces even under serum-free culture. Addition of soluble growth factors to serum-free culture medium effectively induced EC proliferation to confluency. Co-immobilization of cell adhesion peptides and growth factors on thermoresponsive surfaces should be effective for rapid preparation of intact cell sheets and their utilization to regenerative medicine. (c) 2006 Elsevier Ltd. All rights reserved.