Biomimetic Octopus-like Particles for Ultraspecific Capture and Detection of Pathogens.
Biomimetic Octopus-like Particles for Ultraspecific Capture and Detection of Pathogens.
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DOI：
10.1021/acsami.9b05666
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发表时间：
2019-05
影响因子：
9.5
通讯作者：
Dongmei Lv;H. Jiao;Jianwei Dong;Li Sheng;Jinsong Liu;Haisi Dong;Ang Su;Mingjun Zhang;
中科院分区：
文献类型：
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作者：
Dongmei Lv;H. Jiao;Jianwei Dong;Li Sheng;Jinsong Liu;Haisi Dong;Ang Su;Mingjun Zhang;
由病原菌引起的感染性疾病（如败血症、脑膜炎等）严重威胁着公众健康，因此迫切需要快速、准确地鉴定目标菌以预防和治疗细菌感染。虽然已经建立了包括平板计数和聚合酶链反应在内的技术来检测致病菌，但它们要么耗时，要么复杂。本文设计了一种集多臂多价相互作用优点于一体的仿生章鱼结构，用于病原体的超特异性捕获和检测。柔性聚合物臂和多价配体一起工作以模仿章鱼的臂-吸盘协调以有效地抓住目标病原体，导致对目标捕获的显著高的能力和特异性（高于98%，10 CFU mL-1），而没有背景病原体的非特异性吸收。捕获的细菌可以通过表面增强拉曼光谱法鉴定为护理点，检测限为10个细胞mL-1。
Infectious diseases caused by pathogenic bacteria (such as sepsis and meningitis) seriously threaten public health; therefore, rapid and accurate identification of the target bacteria is urgently needed to prevent and treat bacterial infections. Although technologies including plate-counting and polymerase chain reaction have been established to detect the pathogenic bacteria, they are either time-consuming or sophisticated. Herein, a biomimetic octopus-like structure integrating merits of multiarm and multivalent interaction is designed for ultraspecific capture and detection of pathogens. The flexible polymeric arms and multivalent ligands work together to mimic the arm-sucker coordination of an octopus to effectively grasp the target pathogens, leading to remarkably high capacity and specificity for the target capture (above 98%, 10 CFU mL-1) without a nonspecific absorption of background pathogens. The captured bacteria can be identified as a point of care by the surface-enhanced Raman spectroscopy method with a detection limit of 10 cells mL-1.