Precise characterization of human histories in the H2A gene family by top down mass spectrometry
Precise characterization of human histories in the H2A gene family by top down mass spectrometry
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DOI：
10.1021/pr050269n
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Kelleher, NL
中科院分区：
文献类型：
--
作者：
Boyne, MT;Pesavento, JJ;Kelleher, NL
自上而下的分析显示，至少有14个编码组蛋白H2A的基因在HeLa细胞中共表达。对这些物种的特征分析表明，除H2A外，其他所有物种均存在。Z和H2A。F/Z是α - n -乙酰化，H2A。O和h2a、c、D、I、N、P丰度最高，丰度超过10%的缺乏翻译后修饰。这种对H2A形式的明确鉴定说明了自顶向下质谱法的优势，并提供了细胞周期中H2A调控的全局视角。
Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species' revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding similar to 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.