Photoreversal of the ultraviolet radiation-induced disappearance of ATPase-positive Langerhans cells in the epidermis of Monodelphis domestica.
Photoreversal of the ultraviolet radiation-induced disappearance of ATPase-positive Langerhans cells in the epidermis of Monodelphis domestica.
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紫外线辐射诱导的家蝇表皮 ATP 酶阳性朗格汉斯细胞消失的光逆转。
DOI：
10.1002/jlb.44.6.508
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发表时间：
1988
影响因子：
5.5
通讯作者：
Ley,RD
中科院分区：
文献类型：
--
作者：
LeVee,GJ;Applegate,LA;Ley,RD
本研究旨在探讨紫外线辐射(UVR)导致ATPase阳性的表皮朗格汉斯细胞(LC)消失的可能原因。使用家养斑马鱼是因为它具有光重新激活UVR诱导的表皮DNA中的嘧啶二聚体的能力。单独的330J/m2(耳)或500J/m2(背)UVR照射(FS-40太阳灯)减少了ATPase阳性的表皮LC INM的数量。国内约15%的未经辐射的耳朵和约37%的未经辐射的背部皮肤可患此病。UVR术后立即暴露于光复活光(PRL，320-400 nm)90分钟，逆转了UVR的影响，导致ATPase阳性LC数与对照组没有显著差异。暴露于PRL后立即进行UVR并不能阻止ATPase阳性LC的消失。紫外光诱导的ATPase阳性LC消失的光再激活表明DNA损伤(嘧啶二聚体)参与了ATPase阳性LC的消失。
The present study was undertaken to explore the possible causes of ultraviolet radiation (UVR)-induced disappearance of ATPase-positive, epidermal Langerhans cells (LC).Monodelphis domesticawas used because it has the capacity for photoreactivation of UVR-induced pyrimidine dimers in epidermal DNA. Single, 330 J/m2(ears) or 500 J/m2(back) UVR exposures (FS-40 sunlamps) reduced the numbers of ATPase-positive epidermal LC inM. domesticaears to ~15% of those in unirradiated ears and ~37% of those in unirradiated dorsal skin. Immediate 90-minute exposures to photoreactivating light (PRL, 320–400 nm) post-UVR reversed the effects of the UVR, resulting in ATPase-positive LC numbers not being significantly different from controls. Exposure to PRL immediately proceeding UVR did not prevent ATPase-positive LC disappearance. The photoreactivation of UVR-induced ATPase-positive LC disappearance indicates that DNA damage (pyrimidine dimers) is involved in the loss of ATPase-positive LC.