The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.
The intron-containing hsp82 gene of the dimorphic pathogenic fungus Histoplasma capsulatum is properly spliced in severe heat shock conditions.
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二态性病原真菌荚膜组织胞浆菌的含有内含子的hsp82基因在严重的热休克条件下能够正确剪接。
DOI：
10.1128/mcb.11.11.5624-5630.1991
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发表时间：
1991
影响因子：
5.3
通讯作者：
Maresca,B
中科院分区：
文献类型：
--
作者：
Minchiotti,G;Gargano,S;Maresca,B
我们从二型病原真菌荚膜组织胞浆菌（Histoplasma capsulatum）中分离并鉴定了热诱导基因hsp 82。该基因与hsp 82基因家族的其他成员具有高度的同源性，分为3个外显子和2个内含子，分别为122和86个核苷酸。与黑腹果蝇、酿酒酵母和其他生物所证实的相反，H.在温度敏感的唐斯菌株中，在37至40°C的严酷热条件下，荚膜被适当地拼接。在42°C下在耐温G222 B菌株中也观察到剪接准确性，其没有显示初级转录物积累的证据。此外，含有β-微管蛋白基因的内含子也在较高温度范围内正确剪接，这表明剪接中缺乏阻断可能是该生物体中的普遍现象。
We have isolated and characterized a heat-inducible gene,hsp82, from the dimorphic pathogenic fungusHistoplasma capsulatum, which is a filamentous mold at 25°C and a unicellular yeast at 37°C. This gene, which has a high degree of homology with other members of thehsp82gene family, is split into three exons and two introns of 122 and 86 nucleotides, respectively. Contrary to what has been demonstrated inDrosophila melanogaster,Saccharomyces cerevisiae, and other organisms,hsp82mRNA inH. capsulatumis properly spliced during the severe heat conditions of 37 to 40°C in the temperature-sensitive Downs strain. Splicing accuracy was also observed at 42°C in the temperature-tolerant G222B strain, which showed no evidence of accumulation of primary transcripts. Furthermore, the intron containing the β-tubulin gene is also properly spliced at the upper temperature range, suggesting that the lack of a block in splicing may be a general phenomenon in this organism.