Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection
Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection
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DOI：
10.1016/j.vetpar.2011.12.002
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发表时间：
2012-05-25
影响因子：
2.6
通讯作者：
Yu, Hak Sun
中科院分区：
文献类型：
--
作者：
Kang, Shin Ae;Cho, Min Kyoung;Yu, Hak Sun
旋毛虫的感染发生在两个不同的阶段：一个是肠道阶段，另一个是肌肉阶段。评价T.在感染后，我们定期评估感染后小鼠脾细胞的细胞因子产生（AI）。Th 2相关细胞因子水平在T.旋毛虫幼虫肠侵入（1周AI）。Th 2应答的早期升高可能与肠上皮细胞对T.螺旋幼虫入侵。IL-4和IL-13水平在保育细胞形成开始之前（2周AI）达到峰值。此外，除IL-17外，所有Th 17相关细胞因子均略微增加，直至AI 2周。然而，所有的Th 2和Th 17相关的细胞因子的表达水平开始下降后，护士细胞形成的开始，并在4周AI达到基础水平，除了IL-5。同时，CD 4（+）CD 25（+）Foxp 3（+）。脾脏中的T（调节性T，T-reg）细胞群显著增加。此外，外周淋巴结中的细胞数量增加。总之，T.旋毛虫幼虫肠道侵袭诱导Th 2和Th 17细胞相关细胞因子的产生，并随着T-reg细胞相关细胞因子的减少而减少。(C)2011 Elsevier B. V.保留所有权利。
Infection by Trichinella spiralis takes place in two distinct phases: one is the intestinal phase and the other is the muscle phase. To evaluate alterations in cytokine production during a T. spiralis infection, we periodically assessed the cytokine production of splenocytes in mice after infection (AI). The levels of Th2-related cytokines immediately increased after the initiation of T. spiralis larval intestinal invasion (1 week AI). These early elevations in the Th2 response might be associated with the innate immune responses of intestine epithelial cells against T. spiralis larval invasion. IL-4 and IL-13 levels reached a peak prior to the initiation of nurse cell formation (2 weeks AI). Additionally, all Th17-related cytokines, except for IL-17, increased slightly until 2 weeks AI. However, expression levels for all of the Th2 and Th17-related cytokines began to decrease after the initiation of nurse cell formation and reached basal levels at 4 weeks AI, except for IL-5. At the same time, the CD4(+)CD25(+)Foxp3(+). T (regulatory T, T-reg) cell population increased significantly in the spleen. Additionally, the number of cells in the peripheral lymph nodes increased. In conclusion, T. spiral's larva intestinal invasion induced the production of Th2 and Th17 cell-related cytokines, and the cytokines decreased with T-reg cell-related cytokine. (C) 2011 Elsevier B.V. All rights reserved.