Identification of a novel macrophage population in the normal mouse corneal stroma.
Identification of a novel macrophage population in the normal mouse corneal stroma.
复制标题
DOI：
--
复制
发表时间：
2002-07
影响因子：
4.4
通讯作者：
C. Brissette-Storkus;S. Reynolds;A. J. Lepisto;R. Hendricks
中科院分区：
文献类型：
--
作者：
C. Brissette-Storkus;S. Reynolds;A. J. Lepisto;R. Hendricks
目的检测正常小鼠角膜基质中是否存在骨髓源性白细胞。方法用共聚焦显微镜对5 ~ 16周龄正常小鼠的多聚甲醛固定的角膜基质进行单色和双色免疫形态学研究。测定免疫阳性细胞的表型、形态、分布和密度。结果角膜基质中心周围及中央区域有大量的CD 45（+）细胞，形态多样，呈树枝状，细胞密度为200-300个/mm 2。双色免疫组化显示100%的CD 45（+）细胞共表达CD 11b，50%共表达F4/80。约30%的细胞和50%的F4/80（+）细胞共表达主要组织相容性复合物（MHC）II类抗原。表达树突细胞（CD 11 c）或粒细胞（Ly 6 G）标志物的细胞数量非常少至可忽略不计。角膜基质中不存在T细胞和NK细胞的标志物，表明基质中鉴定的所有细胞均为髓系细胞。结论正常小鼠角膜基质中含有大量的CD 45（+）白细胞。大多数这些细胞表达CD 11b标记，但不表达其他树突、粒细胞、T细胞或NK标记，将它们置于单核细胞/巨噬细胞谱系中。
PURPOSE To examine the normal murine corneal stroma for the presence of bone marrow-derived leukocytes. METHODS Wholemounts of paraformaldehyde-fixed corneal stroma from normal mice at 5 to 16 weeks of age were examined in single- and double-color immunomorphologic studies performed with confocal microscopy. The phenotype, morphology, distribution, and density of immunopositive cells were determined. RESULTS Numerous CD45(+) cells with pleomorphic and dendriform morphology were found within the pericentral and central region of the corneal stroma (200-300 cells/mm(2)). Dual-color immunostaining demonstrated that 100% of the CD45(+) cells coexpressed CD11b and 50% coexpressed F4/80. Approximately 30% of the total cells and 50% of the F4/80(+) cells coexpressed major histocompatibility complex (MHC) class II antigens. Very small to negligible numbers of cells expressed markers of dendritic cells (CD11c) or granulocytes (Ly6G). Markers for T-cells and NK cells were absent from the corneal stroma, indicating that all the cells identified in the stroma were of the myeloid lineage. CONCLUSIONS The normal murine corneal stroma contains a significant number of CD45(+) leukocytes. Most these cells express the CD11b marker, but not other dendrite, granulocyte, T-cell, or NK markers, placing them in the monocyte/macrophage lineage.