CHRONIC DIELDRIN EXPOSURE INCREASES HEPATIC DISPOSITION AND BILIARY-EXCRETION OF [C-14] DIELDRIN IN RAINBOW-TROUT
CHRONIC DIELDRIN EXPOSURE INCREASES HEPATIC DISPOSITION AND BILIARY-EXCRETION OF [C-14] DIELDRIN IN RAINBOW-TROUT
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DOI：
10.1006/faat.1993.1039
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发表时间：
1993-04-01
期刊：
影响因子：
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通讯作者：
CURTIS, LR
中科院分区：
文献类型：
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作者：
GILROY, DJ;CARPENTER, HM;CURTIS, LR
先前的工作表明，包括鱼类在内的实验动物接触某些有机氯(OC)杀虫剂会改变随后示踪剂量相同的[14C]OC的组织分布。在目前的研究中，10到20克的彩虹鲑鱼在饲料中暴露于15ppm的狄氏剂。FISH随后每隔两周接受一次攻击，24小时后，腹腔注射0.1 mg/kg狄氏剂和内脏(肝脏、胆汁、肠系膜脂肪、肾脏和肠道)进行放射性分析。狄氏剂处理10周和12周后，在肝脏(200%)、胆汁(500%)和脂肪(10周和12周分别为500%和1200%)中，[14C]-狄氏剂显著高于对照组。其他组织无变化。氯仿/甲醇提取物显示，对照组中对屠体脂肪的标记处置随时间增加，但在经过预处理的鱼中则不是。不能用总体脂的变化或诱导总细胞色素P-450或乙氧基间苯二酚-O-脱乙基酶、戊氧基-鲁芬-O-脱乙基酶、谷胱甘肽转移酶或UDP葡萄糖醛酸基转移酶的活性来解释性情的改变。活体评估显示，20ppm狄氏剂处理9周后，肝脏中的[14C]狄氏剂代谢没有增加，胆汁极浓缩率仅有轻微的增加(22%)。结果表明，肝脏对狄氏剂的隔离、转运或排泄的结构性改变可能是鲑鱼对慢性OC暴露的适应性反应。
Previous work demonstrated that exposure of laboratory animals including fish to certain organochlorine (OC) insecticides altered the tissue distribution of a subsequent tracer dose of the same [14C]OC. In the present study, 10- to 20-g rainbow trout were exposed to 15 ppm dieldrin in the diet. Fish were subsequently challenged at 2-week intervals with an intraperitoneal injection of 0.1 mg/kg [14C]dieldrin and viscera (liver, bile, mesenteric fat, kidney, and intestine) analyzed for radioactivity 24 hr later. After 10 and 12 weeks of dieldrin pretreatment, [14C]-dieldrin was significantly elevated relative to controls in liver (200%), bile (500%), and fat (500 and 1200% for 10 and 12 weeks, respectively) of pretreated fish. Other tissues were unchanged. Chloroform/methanol extractions revealed a time-dependent increase in label disposition to carcass lipid in controls but not in pretreated fish. Altered disposition could not be explained by changes in total body lipid or induction of total cytochrome P-450 or ethoxyresorufin-0-deethylase, pentoxyreso-rufin-O-deethylase, glutathioneS-transferase, or UDP glucuronosyltransferase activities.In vivoassessment of [14C]dieldrin metabolism revealed no increase in hepatic and only a slight (22%) increase in biliary polannonpolar concentration ratio after 9 weeks 20 ppm dieldrin pretreatment. Results suggest that constitutive changes in liver integral to dieldrin sequestration, transport, or excretion may be an adaptive response of trout to chronic OC exposure.