Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells.
Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells.
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DOI：
10.1155/2015/751672
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发表时间：
2015
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
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作者：
He S;Lai R;Chen D;Yan W;Zhang Z;Liu Z;Ding X;Chen Y
口腔鳞状细胞癌（Oral squamous cell carcinoma，OSCC）是口腔癌中最常见的一种，是一种高度恶性的肿瘤，具有高度的局部侵袭和远处转移能力。越来越多的研究表明microRNAs（miRNAs）在调节肿瘤转移和侵袭中发挥重要作用。在本研究中，我们检测miR-221在两个高转移性OSCC细胞系和两个低转移性OSCC细胞系中的表达，使用定量实时PCR分析（qRT-PCR）。qRT-PCR结果表明，miR-221在高转移性OSCC细胞系中上调。然后，通过转染miR-221抑制剂来敲低miR-221表达，并使用transwell迁移和侵袭测定来评估UM 1细胞的迁移和侵袭。结果表明，抑制miR-221抑制了UM 1细胞的迁移和侵袭。此外，甲基-CpG结合域蛋白2（MBD 2）被鉴定为miR-221的直接靶基因。此外，MBD 2沉默可以部分逆转miR-221对细胞迁移和侵袭的影响。总之，下调miR-221至少部分通过靶向人OSCC细胞系UM 1中的MBD 2来抑制细胞迁移和侵袭。
Oral squamous cell carcinoma (OSCC), the most frequent of all oral cancers, is a type of highly malignant tumors with a high capacity to invade locally and form distant metastases. An increasing number of studies have shown that microRNAs (miRNAs) play an important role in regulating cancer metastasis and invasion. In the present study, we detected the expression of miR-221 in two highly metastatic OSCC cell lines and two OSCC cell lines that are less metastatic using quantitative real-time PCR analysis (qRT-PCR). The qRT-PCR results indicate that miR-221 is upregulated in highly metastatic OSCC cell lines. Then, miR-221 expression was knocked down by transfection with miR-221 inhibitor, and UM1 cell migration and invasion were assessed using transwell migration and invasion assays. The results indicate that inhibition of miR-221 suppressed migration and invasion of UM1 cells. Furthermore, methyl-CpG binding domain protein 2 (MBD2) was identified as a direct target gene of miR-221. Additionally, MBD2 silencing could partly reverse the effect of miR-221 on cell migration and invasion. In conclusion, downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1.