Identification and functional analysis of a locus for improvement of lodging resistance in rice
Identification and functional analysis of a locus for improvement of lodging resistance in rice
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DOI：
10.1104/pp.103.029355
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发表时间：
2004-02-01
期刊：
影响因子：
7.4
通讯作者：
Ishimaru, K
中科院分区：
文献类型：
--
作者：
Kashiwagi, T;Ishimaru, K
为了研究水稻抗倒性的提高途径，我们鉴定了一个新的水稻下部抗倒性基因座，并对其生理功能进行了分析。以粳稻日本晴×籼稻Kasalath回交自交系群体为材料，分析了控制水稻下半部抗逆性的数量性状位点（QTL）。共检测到5个抗推性QTL，其中只有第5染色体上的Kasalath（prl5）QTL具有正效应。prl5的似然比曲线与抗台风倒伏的似然比曲线一致。我们选择了三个近等基因系（NILs），其中pr15的染色体区域被取代的Kasalath在日本晴的背景。各近等基因系40 cm以下茎干重、密度和碳水化合物积累量均显著高于日本晴。日本晴和近等基因系在产量、根系特征或上部重量方面没有差异。近等基因系的下部抗推性和抗倒性比日本晴高一倍以上。这些结果表明，pr15可能影响近等基因系下部茎的特性，从而提高抗倒伏性。
We identified a new locus responsible for increased pushing resistance of the lower part of rice (Oryza sativa) and analyzed its physiological function to understand how to improve lodging resistance in rice. Quantitative trait loci (QTLs) controlling pushing resistance of the lower part were analyzed in a population of backcross inbred lines of japonica Nipponbare X indica Kasalath plants cut out at 40 cm to exclude the effect of the weight of the upper parts. Five QTLs for pushing resistance were detected; only one QTL from Kasalath on chromosome 5 (prl5) had a positive effect. The likelihood odds ratio curve of prl5 echoed that for lodging resistance by typhoon. We selected three near-isogenic lines (NILs) in which the chromosomal region of prl5 was substituted with that of Kasalath in the Nipponbare background. The dry weights and densities and the contents of accumulated carbohydrate in stems below 40 cm (lower stems) in each NIL were significantly higher than those of Nipponbare. There was no difference between Nipponbare and the NILs in yield, root characteristics, or the weights of the upper parts. Pushing resistance of the lower part and lodging resistance in the NILs were up to twice as high as in Nipponbare. These results suggest that prl5 might affect the characteristics of the lower stems of the NILs, thus increasing lodging resistance.