Phenolic contents and bioactive potential of peach fruit extracts
Phenolic contents and bioactive potential of peach fruit extracts
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DOI：
10.1016/j.foodchem.2015.12.026
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发表时间：
2016-07-01
期刊：
影响因子：
8.8
通讯作者：
Richard, Tristan
中科院分区：
文献类型：
--
作者：
Mokrani, Abderrahmane;Krisa, Stephanie;Richard, Tristan
对桃果实（Prunus persica L.）的几个品种进行了研究。采用液相色谱-质谱法测定其酚类成分和浓度。浓度以每克干重提取物的毫克数计算。研究了它们的抗氧化能力（Folin-Ciocalteu、ORAC、DPPH、ABTS、PFRAP和ICA）、对β -淀粉样蛋白和α -突触核蛋白纤维形成的抑制性能以及对A - β诱导的PC12细胞系毒性的保护能力（MTT法评估其活力，DCFH-DA法评估细胞内ROS的产生）。鉴定并定量了15种不同的酚类化合物。特别是新的异鼠李素衍生物被鉴定出来。酚含量在19 ~ 82 mg/g之间。春百丽提取物含量最高，罗美亚提取物含量最低。除ICA外，桃果提取物中酚类化合物含量与抗氧化能力之间存在良好的相关性，表明酚类化合物是桃果提取物抗氧化能力的主要影响因素。结果表明，桃品种的酚提取物可抑制A β和α S原纤维的形成，并保护PC12细胞株免受A β诱导的毒性。(C) 2016年Elsevier Ltd.出版
Several cultivars of peach fruit (Prunus persica L.) were investigated. Their phenolic composition and concentration were assessed by LC-MS. Concentrations were calculated in mg per g of dry weight extract. Their antioxidant capacity (Folin-Ciocalteu, ORAC, DPPH, ABTS, PFRAP and ICA), inhibitory property against beta-amyloid and alpha-synuclein fibril formation and protective capacity against A beta-induced toxicity on PC12 cell lines (viability assessed by MTT assay and intracellular ROS production by DCFH-DA assay) were evaluated. Fifteen different phenolic compounds were identified and quantified. In particular, new isorhamnetin derivatives were identified. Phenolic contents were ranged between 19 and 82 mg/g. Spring Belle extract had the highest content and Romea the lowest. Except for the ICA assay, a good correlation between phenolic content and the antioxidant capacities of peach fruit extracts was found, indicating that phenolic compounds are major contributors to their antioxidant capacity. Results indicate that the phenolic extract of peach cultivars inhibits A beta and alpha S fibril formation and protects PC12 cell lines against A beta-induced toxicity. (C) 2016 Published by Elsevier Ltd.