Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana
Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana
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DOI：
10.1007/s00299-011-1042-x
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发表时间：
2011-03
影响因子：
6.2
通讯作者：
Qinghua Wang;Xue Zhang;Fuguang Li;Yuxia Hou;Xingliang Liu;Xueyang Zhang
中科院分区：
文献类型：
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作者：
Qinghua Wang;Xue Zhang;Fuguang Li;Yuxia Hou;Xingliang Liu;Xueyang Zhang
udp -葡萄糖焦磷酸化酶是植物发育的重要调控酶，也是糖原合成的关键酶。本文报道了棉花中UGP全长cDNA的克隆，命名为GhUGP。实时荧光定量PCR分析表明，GhUGP在棉花的根、茎、叶和花中均有表达，其中花和根中表达量较高。GhUGP的转录水平在短时间内依赖于蔗糖和光照，在低温条件下呈上升趋势，在O(2)缺乏条件下呈下降趋势。有趣的是，乙烯诱导后GhUGP在棉花胚珠中的表达显著上调。过表达的GhUGP在拟南芥中表现出不同的表型，与对照相比，其株高和生长率均有所增加。此外，转基因拟南芥可溶性糖、淀粉和纤维素含量增加，但木质素含量没有增加。综上所述，这些结果表明棉花UGPase参与了蔗糖/多糖代谢和细胞壁生物合成，为GhUGP作为促进棉纤维细胞发育的良好候选基因提供了理论推导。
UDP-glucose pyrophosphorylase is an important regulatory enzyme for the development of plants and a critical enzyme in synthesis of glycogen. Here, we reported the cloning of a full-length UGP cDNA from cotton, named GhUGP. Real-time PCR analysis indicated the GhUGP expression in root, stem, leaf and flower of cotton, with a higher level in flower and root. The transcription level of GhUGP depended on sucrose and light in short time and increased under low temperature, but decreased in O(2) deficiency. Interestingly, the expression of GhUGP was significantly up-regulated after ethylene induction in cotton ovules. The over-expression of the GhUGP in Arabidopsis showed discrepant phenotype: increase in height and growth rate when compared with control lines. What is more, the transgenic Arabidopsis had increased contents of soluble sugars, starch and cellulose, but not in lignin content. Collectively, these results indicate that cotton UGPase participates in sucrose/polysaccharides metabolism and cell wall biosynthesis and provide theoretical deduction supporting GhUGP as a good candidate gene for improving the development of cotton fibers cell.