Embryotoxicity of peritoneal fluid in women with endometriosis.: Its relation with cytokines and lymphocyte populations
Embryotoxicity of peritoneal fluid in women with endometriosis.: Its relation with cytokines and lymphocyte populations
复制标题
DOI：
10.1093/humrep/17.3.777
复制
发表时间：
2002-03-01
影响因子：
6.1
通讯作者：
Velasco, I
中科院分区：
文献类型：
--
作者：
Gómez-Torres, MJ;Acién, P;Velasco, I
背景技术背景：本研究的目的是研究子宫内膜异位症或非子宫内膜异位症妇女腹腔液（PF）的胚胎毒性作用，并将任何胚胎毒性与子宫内膜异位症的严重程度、不孕或妊娠的实现、细胞因子浓度和淋巴细胞群相关。方法：66例育龄妇女，54例子宫内膜异位症（21例不孕），12例无子宫内膜异位症不孕，另12例生育妇女作为对照组，被纳入本研究。均在周期后半段行腹腔镜或开腹手术，从道格拉斯囊内采集PF。通过小鼠胚胎试验评估PF的胚胎毒性，并表示为未达到囊胚期的胚胎数量。还研究了PF中存在的细胞因子和淋巴细胞群，并与胚胎毒性相关。结果：PF胚胎毒性在子宫内膜异位症妇女中增加，但与疾病的严重程度几乎没有相关性。然而，尽管没有发现与不孕症存在明确的关系，但在那些后来怀孕的子宫内膜异位症不孕患者中，胚胎毒性似乎较低。我们发现，在高浓度细胞因子的存在下，胚胎毒性显著增加，尤其是白细胞介素-6，而白细胞介素-8则较少（P < 0.05）。没有很好的相关性，观察淋巴细胞群，但CD 56（NK）细胞在PF子宫内膜异位症的妇女显着增加。一般情况下，当PF稀释至20%时，胚胎毒性的相关性更好（91.4 ± 17比68.1 ± 31，P < 0.01）。结论：这些结果表明，在PF细胞因子，特别是IL-6的生产的改变，除了有助于子宫内膜异位症及其演变，可能增加胚胎毒性。然而，没有发现后者与相关不孕症之间的相关性。
BACKGROUND: The goals of the present work were to study the embryotoxic effects of peritoneal fluid (PF) in women with or without endometriosis, and to relate any embryotoxicity to the severity of endometriosis, infertility or achievement of pregnancy, cytokine concentrations and lymphocyte populations. METHODS: Sixty-six consecutive women of reproductive age, 54 with endometriosis (21 infertile) and 12 infertile without endometriosis, and another 12 fertile women as control group, were included in this study. They all underwent laparoscopy or laparotomy in the second half of the cycle, and PF was collected from the pouch of Douglas. The embryotoxicity of the PF was assessed by means of a mouse embryo assay, and expressed as the number of embryos that did not reach blastocyst stage. Cytokines and lymphocyte populations present in PF were also studied and correlated with embryotoxicity. RESULTS: PF embryotoxicity was increased in women with endometriosis, but there was little correlation with the severity of the disease. However, although a clear relationship to the presence of infertility was not found, embryotoxicity appeared to be lower in those infertile patients with endometriosis who later became pregnant. We found a significant increase in embryotoxicity in the presence of high cytokine concentrations, especially with interleukin-6, and less so with interleukin-8 (P < 0.05). No good correlation was observed with lymphocyte populations, but CD56 (NK) cells were significantly increased in the PF of women with endometriosis. In general, the correlations for embryotoxicity were better when PF was diluted at 20% (91.4 ± 17 versus 68.1 ± 31, P < 0.01). CONCLUSIONS: These results suggest that alteration in the production of cytokines in the PF, especially IL-6, besides contributing to the endometriosis and its evolution, probably increases embryoxicity. However, no correlation was found between the latter and associated infertility.