The influence of substrate stiffness gradients on primary human dermal fibroblasts
The influence of substrate stiffness gradients on primary human dermal fibroblasts
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DOI：
10.1016/j.biomaterials.2013.03.075
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Vasilev, Krasimir
中科院分区：
文献类型：
--
作者：
Hopp, Isabel;Michelmore, Andrew;Vasilev, Krasimir
材料的力学性能是细胞增殖、分化和迁移等过程的重要调控因子，近年来受到越来越多的关注。目前，只有很少的方法，薄膜的机械性能可以可控地改变整个表面。在这项工作中，我们提出了一种技术，用于控制产生梯度的表面弹性模量，涉及弱的多层膜（PEM）系统的厚度约为100 nm，并随时间而定的浸泡在1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）作为交联剂的溶液中。通过添加由烯丙胺或丙烯酸的蒸气沉积的10 nm厚的等离子体聚合物层，提供了跨梯度和润湿性的均匀表面化学。我们使用所得的刚度梯度（水合状态下为0.5-110 MPa）来研究人真皮成纤维细胞（HDF）上的粘附、形态和增殖。我们发现，基板的机械性能强烈影响HDF细胞的命运。我们还发现，在本研究中使用的表面性质的实验范围内，与化学和润湿性相比，表面刚度是细胞命运的更强驱动力。(C)2013爱思唯尔有限公司保留所有权利。
Materials mechanical properties are known to be an important regulator of cellular processes such as proliferation, differentiation and migration, and have seen increasing attention in recent years. At present, there are only few approaches where the mechanical properties of thin films can be controllably varied across an entire surface. In this work, we present a technique for controlled generation of gradients of surface elastic moduli involving a weak polyelectrolyte multilayer (PEM) system of approximately 100 nm thickness and time dependent immersion in a solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as a crosslinking agent. Uniform surface chemistry across the gradient and wettability was provided by the addition of a 10 nm thick plasma polymer layer deposited from vapour of either allylamine or acrylic acid. We used the resultant stiffness gradients (0.5-110 MPa in hydrated state) to investigate the adhesion, morphology and proliferation on human dermal fibroblasts (HDFs). We show that substrate mechanical properties strongly influence HDF cell fate. We also found that in the experimental range of surface properties used in this study, the surface stiffness was a stronger driving force to cells fate compared to chemistry and wettability. (C) 2013 Elsevier Ltd. All rights reserved.