A New Method for Cryopreserving Adipose-Derived Stem Cells: An Attractive and Suitable Large-Scale and Long-Term Cell Banking Technology
A New Method for Cryopreserving Adipose-Derived Stem Cells: An Attractive and Suitable Large-Scale and Long-Term Cell Banking Technology
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DOI：
10.1089/ten.tec.2008.0674
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Papaccio, Gianpaolo
中科院分区：
文献类型：
--
作者：
De Rosa, Alfredo;De Francesco, Francesco;Papaccio, Gianpaolo
最近的研究显示了改善干细胞冷冻保存的潜在方法。自体干细胞使用的主要需求是长期储存：这源于人类对未来使用自己细胞的希望。因此，评价冻存前后细胞的活力和分化潜能是非常重要的。虽然有几项研究表明脂肪组织可以长期保存，但其中一些研究将注意力集中在干细胞上。本研究的目的是评估从脂肪组织中收集的冷冻保存的干细胞的命运，并通过最佳的冷冻保存溶液（使用6%的三氯蔗糖，4%的二甲基亚砜和10%的胎牛血清中缓慢冷却）在液氮中低温储存，并开发一种新的方法来有效地保存脂肪来源的干细胞（ASCs），以供未来的临床应用。结果表明，干细胞解冻后仍然能够分化并表达储存前检测到的所有表面抗原，证实了其生物学的完整性。特别是，ASCs分化成脂肪细胞，表现出弥漫性阳性的过氧化物酶体增殖物激活受体γ和脂联素，也能够分化成内皮细胞，而无需添加血管生成因子。因此，ASC可以长期冷冻保存，并且由于其数量庞大，这对于临床应用以及对衍生市场的影响是有吸引力的工具。
Recent studies have shown potential ways for improving stem cell cryopreservation. The major need for autologous stem cell use is a long-term storage: this arises from the humans' hope of future use of their own cells. Therefore, it is important to evaluate the cell potential of vitality and differentiation before and after cryopreservation. Although several studies have shown a long-term preservation of adipose tissue, a few of them focused their attention to stem cells. The aim of this study was to evaluate the fate of cryopreserved stem cells collected from adipose tissue and stored at low a temperature in liquid nitrogen through an optimal cryopreservation solution (using slowly cooling in 6% threalose, 4% dimethyl sulfoxide, and 10% fetal bovine serum) and to develop a novel approach to efficiently preserve adipose-derived stem cells (ASCs) for future clinical applications. Results showed that stem cells, after being thawed, are still capable of differentiation and express all surface antigens detected before storage, confirming the integrity of their biology. In particular, ASCs differentiated into adipocytes, showed diffuse positivity for PPAR gamma and adiponectin, and were also able to differentiate into endothelial cells without addition of angiogenic factors. Therefore, ASCs can be long-term cryopreserved, and this, due to their great numbers, is an attractive tool for clinical applications as well as of impact for the derived market.