Identification of two distinct types of beta-2 microglobulin in marine fish, Pagrus major and Seriola quinqueradiata
Identification of two distinct types of beta-2 microglobulin in marine fish, Pagrus major and Seriola quinqueradiata
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DOI：
10.1016/j.vetimm.2009.09.005
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发表时间：
2010-04-15
影响因子：
1.8
通讯作者：
Aoki, Takashi
中科院分区：
文献类型：
--
作者：
Kondo, Hidehiro;Darawiroj, Damri;Aoki, Takashi
通过肾细胞和脾细胞的表达序列标签 (EST) 分析，在红鲷和黄尾鱼中鉴定出两种类型的 β-2 微球蛋白 (β 2m)。来自红鲷的 21 个 EST 簇与其他硬骨鱼的 β 2m 表现出高度同源性，而另一个包含 7 个 EST 的簇也与 β 2m 同源。同样，含有 21 和 2 个 EST 的两个簇被鉴定为与黄尾鱼中的 β 2m 同源。每条鱼的大簇和小簇中的基因分别指定为β 2m-1 和β 2m-2。虽然β 2m-1s 的氨基酸序列与其他硬骨鱼中鉴定的氨基酸序列高度同源，但β 2m-2s 的氨基酸序列与鱼类β 2m 的同源性较低。红鲷的β2m-1缺乏脊椎动物中观察到的两个内含子中的第一个内含子，而β2m-2则缺乏两个内含子。脊椎动物 β 2m 蛋白的系统发育分析表明，海鱼中的 β 2m-2 与其他硬骨鱼中的 β 2m 不同。 (C) 2009 Elsevier B.V. 保留所有权利。
Two types of beta-2 microglobulin (beta 2m) were identified in red seabream and yellowtail from expressed sequence tag (EST) analyses of kidney and spleen cells. A cluster of 21 ESTs from red seabream showed high homology to beta 2m from other teleosts, while another cluster containing 7 ESTs was also homologous to beta 2m. Similarly, two clusters containing 21 and 2 ESTs were identified to be homologous to beta 2m in yellowtail. The genes in the large and small clusters of each fish were designated beta 2m-1 and beta 2m-2, respectively. Although the amino acid sequences of beta 2m-1s were highly homologous to those identified in the other teleosts, those of beta 2m-2s were less homologous to fish beta 2m. beta 2m-1 of red seabream lacks the first intron of two introns observed in vertebrtes, and beta 2m-2 lacks both introns. Phylogenetic analysis of vertebrate beta 2m proteins suggested that the beta 2m-2 in marine fish are distinct from beta 2m in the other teleosts. (C) 2009 Elsevier B.V. All rights reserved.