Radial P34HB Electrospun Fiber: A Scaffold for Bone Tissue Engineering
Radial P34HB Electrospun Fiber: A Scaffold for Bone Tissue Engineering
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径向 P34HB 静电纺纤维：骨组织工程的支架
DOI：
10.1166/jnn.2020.18583
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发表时间：
2020-10-01
影响因子：
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通讯作者：
Cai, Xiaoxiao
中科院分区：
文献类型：
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作者：
Fu, Na;Meng, Zhaosong;Cai, Xiaoxiao
寻找合适的骨组织工程支架材料一直是骨组织工程研究的一个巨大挑战。设计和制备具有仿生性能的骨支架也是骨修复工程的难题。本项目旨在通过静电纺丝技术制备径向生物塑料聚-3-羟基丁酸酯-4-羟基丁酸酯（P34 HB）静电纺丝纤维支架，其模拟结构，组成和刚度特性。采用接触角仪和万能材料试验机对径向纤维支架的表面形貌、亲水性进行了测试。荧光显微镜下观察骨髓间充质干细胞（BMSC）在放射状P34 HB电纺纤维支架上的细胞形态。在径向P34 HB静电纺丝纤维支架上进行细胞活力测试。我们进一步测试了放射状纤维支架的成骨分化能力。结果表明，放射状P34 HB电纺纤维支架具有良好的生物安全性、生物相容性和成骨诱导性。支架的放射状结构对骨形成的诱导也有很强的影响。此外，该结构还可以改善植入物的骨接触面积，并增加植入物与骨之间的锁定和固定。我们计划将此方法应用于骨缺损修复的动物实验中进行进一步的研究。
Finding suitable scaffold material is always an enormous challenge in the study of bone tissue engineering. Designing and preparing bone scaffolds with biomimetic properties is also a difficult problem for bone reparation projects. This project intends to fabricate radial bio-plasticpoly-3-hydroxybutyrate-4-hydroxybutyrate (P34HB) electrospun fibers scaffold that mimic structural, compositional and stiffness properties via an electro spinning technique. The surface morphology, hydrophilicity of the radial fibers scaffold were tested, a contact angle meter and a universal material tester. Bone marrow mesenchymal stem cell (BMSC) morphologies on radial P34HB electrospun fibers scaffold were observed after cell culture under fluorescence microscopy. Tests of cell viability on radial P34HB electrospun fibers scaffold were conducted. We further tested the osteogenic differentiation ability of radial fibers scaffold. These results showed that radial P34HB electrospun fibers scaffold have good biosafety, biocompatibility and osteogenic induction. The radial structure of the scaffold also has a strong effect on the induction of bone formation. Moreover, the structure could also improve the bone contact area of the implant and increase the locking and fixation between the implant and the bone. We plan to apply this procedure to animal experiments for bone defect repair in further research.