Photographic and spectroscopic correlations of human cataracts.
Photographic and spectroscopic correlations of human cataracts.
复制标题
人类白内障的摄影和光谱相关性。
DOI：
10.1159/000266762
复制
发表时间：
1989
影响因子：
2.1
通讯作者：
Matthews,N
中科院分区：
文献类型：
--
作者：
Lerman,S;Moran,M;Matthews,N
手术摘除后3小时内获得的囊内白内障用CCRG技术拍照，并立即进行荧光光谱分析，随后进行31 P和13 C核磁共振（NMR）光谱分析。荧光光谱显示非色氨酸荧光强度和透镜颜色之间具有良好的相关性。还观察到由内在透镜色氨酸荧光的290 nm激发峰确定的光散射程度与这些白内障晶状体的CCRG（摄影）外观之间存在有趣的相关性。基于对100例白内障的分析，这种光散射测量与透镜混浊的类型和程度之间存在很强的相关性。类似的相关性是显而易见的，与31磷核磁共振有机磷酸盐档案中的晶体，其中磷酸糖水平升高，只有在糖尿病患者的白内障（'糖尿病性白内障'）。除了荧光和31 P-NMR光谱法之外，所选晶状体也在获得后立即与5.5 nM 13 C-葡萄糖一起孵育，并进行前述光谱法，随后进行13 C-NMR分析以检测和监测年轻与老年正常晶状体和糖尿病性白内障中的山梨醇积累。这些研究清楚地证明了非色氨酸荧光发色团水平、光散射（由色氨酸激发峰确定）、透镜年龄和白内障类型之间的直接相关性。此外，有机磷谱清楚地描述了糖尿病性白内障，13 C-NMR谱与年龄相关的醛糖还原酶活性降低相关
Intracapsular cataracts obtained within 3 h after surgical extraction were photographed with the CCRG technique and immediately subjected to fluorescence spectroscopy followed by31P- and13C-nuclear magnetic resonance (NMR) spectroscopy. Fluorescence spectroscopy demonstrates an excellent correlation between nontryptophan fluorescence intensities and lens color. An interesting correlation was also observed between the degree of light scatter as determined by the 290-nm excitation peak for intrinsic lens tryptophan fluorescence and the CCRG (photographic) appearance of these cataractous lenses. Based on 100 cataracts analyzed, there is a strong correlation between this kind of light scattering measurement and the type and degree of lens opacification. A similar correlation is evident with the31P-NMR organophosphate profiles in the lenses in which the sugar phosphate levels are elevated only in diabetic patients with cataracts (‘diabetic cataracts’). Aside from fluorescence and31P-NMR spectroscopy, selected lenses were also incubated with 5.5 nM13C-glucose as soon as they were obtained, and the foregoing spectroscopy was performed, followed by13C-NMR analyses to detect and monitor for sorbitol accumulation in young versus old normal lenses and in diabetic cataracts. These studies clearly demonstrate a direct correlation between nontryptophan-fluorescent chromophore levels, light scattering (determined by tryptophan excitation peaks), lens age and cataract type. In addition, the organophosphate profiles clearly delineate the diabetic cataracts, and the13C-NMR spectra correlate well with the age-related decrease in aldose reductase activity