Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration
Comparative evaluation of MSCs from bone marrow and adipose tissue seeded in PRP-derived scaffold for cartilage regeneration
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来自骨髓和脂肪组织的 MSC 接种在 PRP 衍生支架中用于软骨再生的比较评估
DOI：
10.1016/j.biomaterials.2012.06.058
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发表时间：
2012-10-01
期刊：
影响因子：
14
通讯作者：
Zhang, Changqing
中科院分区：
文献类型：
--
作者：
Xie, Xuetao;Wang, Yang;Zhang, Changqing
本研究的目的是：(1)确定是否可以制备富血小板血浆（PRP）作为一种具有生物活性的支架，能够释放内源性生长因子用于软骨修复；(2)比较PRP支架内骨髓间充质干细胞（BMSC）和脂肪间充质干细胞（ADSC）的成软骨分化能力；(3)在体内测试ADSC-PRP构建物对软骨再生的效果。体外评价表明，PRP可形成网状结构的三维支架，具有内源性生长因子释放能力和细胞整合能力。在PRP支架中播种后，BMSC比ADSC具有更高的增殖率和软骨特异性基因和蛋白的表达。在兔骨软骨缺损模型中，PRP支架内植入的骨髓间充质干细胞也表现出更好的大体外观、组织学和免疫组织化学特征、更高的软骨特异性基因和蛋白表达以及软骨下骨再生。植入后9周，ADSC种子构建物在家兔体内发育成分泌软骨基质的功能性软骨细胞。我们的研究结果表明，PRP是一种能够释放内源性生长因子的候选生物活性支架，并且PRP支架内植入的BMSC和ADSC分化为软骨细胞，可能适用于基于细胞的软骨修复。(C) 2012 Elsevier Ltd.版权所有。
The aims of this study were to (1) determine whether platelet-rich plasma (PRP) could be prepared as a bioactive scaffold capable of endogenous growth factor release for cartilage repair; (2) compare the chondrogenic differentiation ability of mesenchymal stem cells (MSCs) from bone marrow (BMSC) and from adipose (ADSC) seeded within the PRP scaffold; and (3) test the efficacy of ADSC-PRP construct in cartilage regeneration in vivo. In vitro evaluation showed that a 3-dimensional scaffold with a mesh-like microstructure was formed from PRP, with the capability of endogenous growth factor release and ready cell incorporation. Upon seeding in the PRP scaffold, BMSC showed higher proliferation rate, and higher expression of cartilage-specific genes and proteins than ADSC. In an osteochondral defect model in rabbits, implanted BMSC seeded within PRP scaffold also exhibited better gross appearance and histological and immunohistochemical characteristics, higher cartilage-specific gene and protein expression as well as subchondral bone regeneration. ADSC seeded constructs developed into functional chondrocytes secreting cartilaginous matrix in rabbits at 9 weeks post-implantation. Our findings suggest that PRP is a candidate bioactive scaffold capable of releasing endogenous growth factors and that BMSC and ADSC seeded within the PRP scaffold differentiate into chondrocytes and may be suitable for cell-based cartilage repair. (C) 2012 Elsevier Ltd. All rights reserved.