Expansion of mesenchymal stem cells on fibrinogen-rich protein surfaces derived from blood plasma
Expansion of mesenchymal stem cells on fibrinogen-rich protein surfaces derived from blood plasma
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DOI：
10.1002/term.352
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发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Frisbie, David D.
中科院分区：
文献类型：
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作者：
Kisiday, John D.;Hale, Benjamin W.;Frisbie, David D.
骨髓间充质干细胞（MSCs）存在于低密度的骨髓中，培养扩增对于获得足够数量的骨髓间充质干细胞是必要的。研究人员已经发现MSC在组织培养塑料（TCP）上生长，尽管很少有研究探索在其他生长基质上的增殖。利用成年马间充质干细胞，我们评估了血浆生成的富纤维蛋白原沉淀（FRP）表面的增殖情况。当以1 × 10(4)个细胞/厘米(2)的速度播种并在10天内传代5次时，在含有成纤维细胞生长因子2 （FGF2）的培养基中，玻璃钢上的MSCs的细胞产量相对于TCP增加了2.5倍。在不含FGF2的培养基中，10天后，FRP刺激的细胞产量比TCP增加了10.4倍，尽管在FGF2中保持TCP的对照培养表明FRP的刺激效果不如FGF2持久。软骨培养表明FRP不影响分化。在TCP上，经过10天的培养，500个细胞/cm(2)的MSCs的细胞产量比1 × 10(4)个细胞/cm(2)的MSCs增加了4.6倍。在500个细胞/厘米(2)的培养中，玻璃钢刺激细胞产量比TCP增加两倍，而不影响分化。低密度玻璃钢培养比TCP显示出更均匀的细胞分布，这表明玻璃钢可能通过减少减慢增殖的接触抑制来加速增殖。此外，FRP似乎能够结合FGF2，因为用FGF2预处理的FRP表面比不含FGF2的培养物支持更大的增殖。综上所述，这些因素表明，从简单廉价的血液处理中获得的底物可增强间充质干细胞的增殖，并促进膨胀表面的有效覆盖。版权所有John Wiley & Sons， Ltd。
Mesenchymal stem cells (MSCs) are present in low density in bone marrow and culture expansion is necessary to obtain sufficient numbers for many proposed therapies. Researchers have characterized MSC growth on tissue culture plastic (TCP), although few studies have explored proliferation on other growth substrates. Using adult equine MSCs, we evaluated proliferation on fibrinogen-rich precipitate (FRP) surfaces created from blood plasma. When seeded at 1 x 10(4) cells/cm(2) and passaged five times over 10 days, MSCs on FRP in medium containing fibroblast growth factor 2 (FGF2) resulted in a similar to 2.5-fold increase in cell yield relative to TCP. In FGF2-free medium, FRP stimulated a 10.4-fold increase in cell yield over TCP after 10 days, although control cultures maintained in FGF2 on TCP demonstrated that the stimulatory effect of FRP was not as lasting as that of FGF2. Chondrogenic cultures demonstrated that FRP did not affect differentiation. On TCP, MSCs seeded at 500 cells/cm(2) experienced a 4.6-fold increase in cell yield over cultures seeded at 1 x 10(4) cells/cm(2) following 10 days of expansion. In 500 cells/cm(2) cultures, FRP stimulating a two-fold increase in cell yield over TCP without affecting differentiation. Low-density FRP cultures showed a more even distribution of cells than TCP, suggesting that FRP may accelerate proliferation by reducing contact inhibition that slows proliferation. In addition, FRP appears capable of binding FGF2, as FRP surfaces pre-conditioned with FGF2 supported greater proliferation than FGF2-free cultures. Taken together, these factors indicate that substrates obtained from simple and inexpensive processing of blood enhance MSC proliferation and promote efficient coverage of expansion surfaces. Copyright (C) 2010 John Wiley & Sons, Ltd.