Effect of G-CSF on the osteoinductive property of a BCP/blood clot composite
Effect of G-CSF on the osteoinductive property of a BCP/blood clot composite
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DOI：
10.1002/jbm.a.35424
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发表时间：
2015-09-01
影响因子：
4.9
通讯作者：
Rochet, Nathalie
中科院分区：
文献类型：
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作者：
Paul, Adrien J.;Momier, David;Rochet, Nathalie
我们之前报道过，血凝块与双相磷酸钙微粒结合构成了一种生物材料（BRB），可以修复大鼠的骨严重缺损并诱导小鼠皮下骨形成。粒细胞集落刺激因子 (G-CSF) 是人类最常使用的一种试剂，用于丰富血液中的造血干细胞和祖细胞 (HSPC) 以及粒细胞 (GC)。此外，最近的数据还表明它可以动员间充质干细胞（MSC）。在这里，我们询问 G-CSF 是否可以通过用 G-CSF 动员的血液 (BRBe) 替代正常血液或通过用 G-CSF 治疗受体动物来进一步增强 BRB 的骨诱导特性。在 C57BL/6 小鼠中进行的实验表明，G-CSF 会诱导循环 HPC 和 GC 的显着增加，但不会导致 MSC 的显着增加。与用正常血液制备的BRB相比，用富含G-CSF的血液制备的BRBe诱导皮下骨形成轻微但显着的减少。向受体小鼠额外注射G-CSF对BRB或BRBe诱导的骨形成没有显着影响。总之，这些结果表明，在异位植入模型中，G-CSF 诱导的细胞动员对该血液/BCP 复合材料的骨诱导特性具有负面影响。 (C) 2015 年 Wiley 期刊公司。
We previously reported that blood clot combined with biphasic calcium phosphate microparticles constitute a biomaterial (BRB) that can repair a bone critical defect in rat and induces subcutaneous bone formation in mice. The granulocyte colony-stimulating factor (G-CSF) is the agent most commonly used in human to enrich blood with hematopoietic stem and progenitor cells (HSPCs) as well as granulocytes (GCs). Moreover, recent data also suggest that it can mobilize mesenchymal stem cells (MSCs). Here, we asked whether the osteoinductive properties of the BRB could be further enhanced by G-CSF, either by replacing normal blood by G-CSF-mobilized blood (BRBe) or by treating the recipient animals with G-CSF. The experiments performed in C57BL/6 mice showed that G-CSF induces a marked increase of circulating HPCs and GCs, but not of MSCs. BRBe prepared with G-CSF-enriched blood induced a slight but significant decrease of subcutaneous bone formation compared to BRB prepared with normal blood. Additional injection of G-CSF to the recipient mice had no significant effect on the bone formation induced by BRB or BRBe. Altogether these results indicate that, in this model of ectopic implantation, cell mobilization induced by G-CSF has a negative effect on the osteoinductive property of this blood/BCP composite. (C) 2015 Wiley Periodicals, Inc.