Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus S locus
Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus S locus
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DOI：
10.1007/s00497-008-0069-9
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发表时间：
2008-06-01
影响因子：
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通讯作者：
Tao, Ryutaro
中科院分区：
文献类型：
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作者：
Matsumoto, Daiki;Yamane, Hisayo;Tao, Ryutaro
蔷薇科李属的S基因座及其侧翼区域含有4个花粉表达的F-box基因。这些基因包括具有低等位基因序列多态性的S座位F-box基因1、2和3(SLFl1、SLFl2和SLFl3)以及可能的花粉S基因，命名为S单倍型特有的F-box蛋白基因。由于对SLFL功能的研究比SFB少得多，本研究对6个S单倍型甜樱桃的SLFL进行了分析。基因组DNA印迹分析和SLFl1基因的分离表明，功能自交不亲和的S-3单倍型的SLFl1基因缺失，在S-3基因座区域只剩下一个与SLFl1相似的部分序列，这表明SLFl1本身并不直接参与花粉管的生长和GSI反应。基因组DNA印迹分析表明，SLFl2和SLFl3没有实质性的修饰或突变。对玫瑰科S座位及其邻近地区F-box基因的系统发育分析表明，李的F-box基因与S座位F-box兄弟的亲缘关系比与李的亲缘关系更近。在此基础上讨论了自交不亲和性的进化和SLFL的功能。
The S locus and its flanking regions in the genus Prunus (Rosaceae) contain four pollen-expressed F-box genes. These genes contain the S locus F-box genes with low allelic sequence polymorphism genes 1, 2, and 3 (SLFL1, SLFL2, and SLFL3) as well as the putative pollen S gene, named the S haplotype-specific F-box protein gene (SFB). As much less information is available on the function of SLFLs than that of SFB, we analyzed the SLFLs of six S haplotypes of sweet cherry (Prunus avium) in this study. Genomic DNA blot analysis and the isolation of SLFL1 showed that the SLFL1 gene in a functional self-incompatible S-3 haplotype is deleted and only a partial sequence resembling SLFL1 is left in the S-3 locus region, suggesting that SLFL1 by itself is not directly involved in either the GSI reaction or pollen-tube growth. Genomic DNA blot analysis showed that there was no substantial modification or mutation in SLFL2 and SLFL3. A phylogenic analysis of F-box genes in the rosaceous S locus and its border regions showed that Prunus SLFLs were more closely related to maloid S locus F-box brothers than to Prunus SFBs. The functions of SLFLs and the evolution of self-incompatibility in Prunus are discussed based on these results.