Golgi-like labeling of a single neuron recorded extracellularly
Golgi-like labeling of a single neuron recorded extracellularly
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DOI：
10.1016/0304-3940(94)90332-8
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发表时间：
1994-04
影响因子：
2.5
通讯作者：
D. Pinault
中科院分区：
文献类型：
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作者：
D. Pinault
我们描述了一种新的和强大的细胞外的方法染色的一个单一的神经元确定的电生理标准。该单单元技术涉及使用玻璃微电极（尖端直径：1.5-2.5 μm），电极内填充含1.5%生物胞素或神经生物素的盐溶液（NaCl; 0.5 M）。一旦神经元被记录、分离和识别，示踪剂就由几nA的阳极电流脉冲递送。在排出过程中，细胞必须保持良好的分离和存活，以确保最佳染色。有证据表明，被标记的神经元实际上是被记录的神经元。我们的方法简单可靠，成功率超过85%。讨论了其优点和缺陷。这种单单位标记技术可以进一步与从生物学到行为学研究的任何其他程序相结合。
We describe a novel and powerful extracellular method for the staining of a single neuron identified by electrophysiological criteria. This single-unit technique involves the use of glass micro-electrodes (tip diameter: 1.5–2.5 μm) filled with a saline solution (NaCl; 0.5 M) containing 1.5% of biocytin or Neurobiotin. Once a neuron is recorded, isolated and identified, the tracer is delivered by anodal current pulses of a few nA. The cell must remain well isolated and alive during the ejection procedure to ensure optimal staining. Evidence is provided that the labeled neuron is actually the one that was recorded. Our simple method is reliable with a success rate exceeding 85%. The advantages and pitfalls are discussed. This single-unit labeling technique could further be combined with any other procedures ranging from biological to behavioral studies.