Oxidized glutathione and uric acid as biomarkers of early cystic fibrosis lung disease
Oxidized glutathione and uric acid as biomarkers of early cystic fibrosis lung disease
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DOI：
10.1016/j.jcf.2016.10.012
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发表时间：
2017-03-01
影响因子：
5.2
通讯作者：
Kettle, Anthony J.
中科院分区：
文献类型：
--
作者：
Dickerhof, Nina;Turner, Rufus;Kettle, Anthony J.
背景资料：在囊性纤维化（CF）中，迫切需要使用基于血液和尿液的生物标志物来早期诊断肺部感染和炎症。方法：使用质谱法，在来自36名患有CF的婴儿和儿童的支气管肺泡灌洗液（BAL）、血清和尿液的匹配样本中测量谷胱甘肽和尿酸的氧化产物，并将其与BAL中的嗜酸性炎症和感染的标志物相关。与未感染铜绿假单胞菌（PsA）的儿童相比，BAL中谷胱甘肽（谷胱甘肽磺酰胺，GSA）和尿酸（尿囊素）的氧化产物升高（p < 0.05），并与其他嗜酸性炎症标志物相关。PsA感染患儿血清GSA明显升高（P < 0.01）。尿GSA与肺GSA（r = 0.42，p < 0.05）和嗜中性粒细胞炎症标志物相关。结论：这项概念验证研究表明，尿GSA而非尿囊素有望作为CF肺疾病早期嗜中性粒细胞炎症的非侵入性标志物。(C)2016年欧洲囊性纤维化协会。Elsevier B.V.出版，保留所有权利。
Background: In cystic fibrosis (CF) there is an urgent need for earlier diagnosis of pulmonary infections and inflammation using blood-and urine based biomarkers.Methods: Using mass spectrometry, oxidation products of glutathione and uric acid were measured in matched samples of bronchoalveolar lavage (BAL), serum and urine from 36 infants and children with CF, and related to markers of neutrophilic inflammation and infection in BAL.Results: Oxidation products of glutathione (glutathione sulfonamide, GSA) and uric acid (allantoin), were elevated in BAL of children with pulmonary infections with Pseudomonas aeruginosa (PsA) compared to those without (p < 0.05) and correlated with other markers of neutrophilic inflammation. Serum GSA was significantly elevated in children with PsA infections (p < 0.01). Urinary GSA correlated with pulmonary GSA (r = 0.42, p < 0.05) and markers of neutrophilic inflammation.Conclusions: This proof-of-concept study demonstrates that urinary GSA but not allantoin shows promise as a non-invasive marker of neutrophilic inflammation in early CF lung disease. (C) 2016 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.