Imaging trace element distributions in single organelles and subcellular features.
Imaging trace element distributions in single organelles and subcellular features.
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DOI：
10.1038/srep21437
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发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
El-Muayed M
中科院分区：
文献类型：
--
作者：
Kashiv Y;Austin JR 2nd;Lai B;Rose V;Vogt S;El-Muayed M
细胞内化学元素的分布在广泛的基础和应用生化研究中至关重要。一个例子是亚细胞锌分布在产生胰岛素的胰腺β细胞中锌稳态和2型糖尿病发展中的作用。据我们所知，我们将透射电子显微镜与微米和纳米同步加速器 X 射线荧光相结合，首次对单个细胞器和其他亚细胞特征中的自然元素分布（包括微量元素的分布）进行明确成像。检测到的元素包括 Cl、K、Ca、Co、Ni、Cu、Zn 和 Cd（某些细胞补充了这些元素）。细胞样品的制备技术对自然元素浓度和分布影响最小，且不使用荧光指示剂。它可能适用于所有细胞类型，并在细胞器群体水平研究中无法提供的单一细胞器水平上提供新的生化见解。
The distributions of chemical elements within cells are of prime importance in a wide range of basic and applied biochemical research. An example is the role of the subcellular Zn distribution in Zn homeostasis in insulin producing pancreatic beta cells and the development of type 2 diabetes mellitus. We combined transmission electron microscopy with micro- and nano-synchrotron X-ray fluorescence to image unequivocally for the first time, to the best of our knowledge, the natural elemental distributions, including those of trace elements, in single organelles and other subcellular features. Detected elements include Cl, K, Ca, Co, Ni, Cu, Zn and Cd (which some cells were supplemented with). Cell samples were prepared by a technique that minimally affects the natural elemental concentrations and distributions, and without using fluorescent indicators. It could likely be applied to all cell types and provide new biochemical insights at the single organelle level not available from organelle population level studies.