A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population
A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population
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DOI：
10.1128/jb.185.2.654-659.2003
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发表时间：
2003-01-01
影响因子：
3.2
通讯作者：
Zgur-Bertok, D
中科院分区：
文献类型：
--
作者：
Mulec, J;Podlesek, Z;Zgur-Bertok, D
在原核生物中，仅描述了细胞群体中差异基因表达的少数实例。大肠杆菌的天然群体中的大肠杆菌素生产，虽然在自然栖息地提供了竞争优势，但也导致产毒素细胞的裂解。已经发现大肠杆菌素K的合成由于ppGpp的增加而被诱导（I. Kuhar，J. P.货车Putten，D.兹古尔-贝尔托克Gaastra和B。J. Jordi，Mol. Microbiol. 41：207-216）。使用两个转录融合，cka-gfp和cki-gfp，我们表明，在单细胞水平上，大肠杆菌素K活性基因cka的表达在只有3%的细菌种群诱导营养饥饿后。相反，免疫基因cki在大多数细胞中表达。在lexA缺陷型菌株和relA spoT突变型菌株中cka-gfp融合体的表达表明cka的差异表达主要在转录水平上建立。
In prokaryotes, only a few examples of differential gene expression in cell populations have been described. Colicin production in natural populations of Escherichia coli, while providing a competitive advantage in the natural habitat, also leads to lysis of the toxin-producing cell. Colicin K synthesis has been found to be induced due to an increase in ppGpp (I. Kuhar, J. P. van Putten, D. Zgur-Bertok W. Gaastra, and B. J. Jordi, Mol. Microbiol. 41:207-216). Using two transcriptional fusions, cka-gfp and cki-gfp, we show that at the single-cell level, the colicin K activity gene cka is expressed in only 3% of the bacterial population upon induction by nutrient starvation. In contrast, the immunity gene cki is expressed in the large majority of the cells. Expression of the cka-gfp fusion in a lexA-defective strain and in a relA spoT mutant strain indicates that differential expression of cka is established primarily at the level of transcription.