Luminescent detection of the lipopolysaccharide endotoxin and rapid discrimination of bacterial pathogens using cationic platinum(II) complexes
Luminescent detection of the lipopolysaccharide endotoxin and rapid discrimination of bacterial pathogens using cationic platinum(II) complexes
复制标题
使用阳离子铂(II)络合物发光检测脂多糖内毒素并快速辨别细菌病原体
DOI：
10.1039/c7ra03312e
复制
发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Yu Bingran
中科院分区：
文献类型：
--
作者：
Zhu Yiwen;Xu Chen;Wang Yu;Chen Yaqing;Ding Xiaokang;Yu Bingran
报道了一种基于2，6-二(苯并咪唑-2‘-基)吡啶与六甘醇甲醚([Pt(N^N^N)Cl]+)的氯铂(II)络合物的发光探针，用于检测内毒素和快速区分革兰氏阴性和革兰氏阳性细菌。[PT(N^N^N)Cl]+可溶于水溶液，发光强度最小。在内毒素存在下，[铂(N^N^N)Cl]+与带负电荷的内毒素结合形成内毒素-铂(II)聚集体。脂多糖-铂(II)聚集体的形成增强了分子间的铂、⋯、铂和π-π的堆积相互作用，从而导致了三重态金属-金属-配体电荷转移(3MMLCT)导致以650 nm为中心的发光。最低检测限(LOD)为5.7 nM。我们还展示了一种概念验证应用[PT(N^N^N)Cl]+，用于在5分钟内快速且免洗涤地区分革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌。[Pt(N^N^N)Cl]+的上述特性使其在细菌内毒素的检测和细菌病原体的识别方面具有良好的临床应用前景。
A luminescence probe based on chloroplatinum(II) complexes of 2,6-bis(benzimidazol-2′-yl)pyridine with hexaethylene glycol methyl ether groups ([Pt(N^N^N)Cl]+) was reported for sensing of the lipopolysaccharide (LPS) endotoxin and rapid discrimination of Gram-negative and Gram-positive bacterial pathogens. [Pt(N^N^N)Cl]+ can be dissolved in aqueous solution with minimal luminescence emission. In the presence of LPS, [Pt(N^N^N)Cl]+ binds to negatively charged LPS to form LPS–Pt(II) aggregates. The formation of LPS–Pt(II) aggregates enhances the intermolecular Pt⋯Pt and π–π stacking interactions, and consequently leads to luminescence emission centered at 650 nm due to triplet metal-to-metal-to-ligand charge-transfer (3MMLCT). The limit of detection (LOD) of LPS is 5.7 nM. We also demonstrate a proof-of-concept application of [Pt(N^N^N)Cl]+ for rapid and washing-free discrimination of Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus within 5 min. The above features of [Pt(N^N^N)Cl]+ make it a promising sensor for clinical applications in the detection of endotoxins and discrimination of bacterial pathogens.