Characterization of a cDNA encoding a protein involved in formation of the skeleton during development of the sea urchin Lytechinus pictus.
Characterization of a cDNA encoding a protein involved in formation of the skeleton during development of the sea urchin Lytechinus pictus.
复制标题
编码参与海胆 Lytechinus pictus 发育过程中骨骼形成的蛋白质的 cDNA 的表征。
DOI：
10.1016/0012-1606(91)90265-5
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
Wilt,F
中科院分区：
文献类型：
--
作者：
Livingston,BT;Shaw,R;Bailey,A;Wilt,F
为了研究蛋白质在发育过程中矿化组织形成中的作用，我们分离出了一种编码蛋白质的cDNA，该蛋白质是海胆（Lytechinus pictus）骨骼骨针有机基质的一个组成部分。编码这种蛋白质的RNA的表达在发育过程中受到调节，并定位于微球谱系的后代。该cDNA序列与其他种类的海胆的同源cDNA的比较显示，该蛋白质是基本的，并包含三个保守的结构基序：信号肽，脯氨酸丰富的区域，和一个不寻常的区域组成的一系列直接重复。对该cDNA编码的蛋白质的研究证实了从核苷酸序列推导的预测阅读框架，并表明该蛋白质是分泌的，没有糖基化。氨基酸序列与数据库的比较显示，重复结构域与形成独特β-螺旋超二级结构的蛋白质相似。
In order to investigate the role of proteins in the formation of mineralized tissues during development, we have isolated a cDNA that encodes a protein that is a component of the organic matrix of the skeletal spicule of the sea urchin,Lytechinus pictus. The expression of the RNA encoding this protein is regulated over development and is localized to the descendents of the micromere lineage. Comparison of the sequence of this cDNA to homologous cDNAs from other species of urchin reveal that the protein is basic and contains three conserved structural motifs: a signal peptide, a proline-rich region, and an unusual region composed of a series of direct repeats. Studies on the protein encoded by this cDNA confirm the predicted reading frame deduced from the nucleotide sequence and show that the protein is secreted and not glycosylated. Comparison of the amino acid sequence to databases reveal that the repeat domain is similar to proteins that form a unique β-spiral supersecondary structure.