Axil, a Member of the Axin Family, Interacts with Both Glycogen Synthase Kinase 3β and β-Catenin and Inhibits Axis Formation ofXenopus Embryos
Axil, a Member of the Axin Family, Interacts with Both Glycogen Synthase Kinase 3β and β-Catenin and Inhibits Axis Formation ofXenopus Embryos
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Axil 是 Axin 家族的成员，与糖原合酶激酶 3β 和 β-连环蛋白相互作用并抑制非洲爪蟾胚胎的轴形成
DOI：
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发表时间：
1998
影响因子：
5.3
通讯作者：
A. Kikuchi
中科院分区：
文献类型：
--
作者：
Hideki Yamamoto;S. Kishida;T. Uochi;S. Ikeda;S. Koyama;M. Asashima;A. Kikuchi
摘要利用酵母双杂交技术，我们鉴定了一个与糖原合成酶激酶3β（GSK-3β）相互作用的新蛋白。该蛋白与Wnt信号通路的负调控因子Axin具有44%的氨基酸同源性，我们将其命名为Axin like Axil。像Axin一样，Axil使爪蟾胚胎腹侧化并抑制Xwnt 8诱导的爪蟾轴复制。Axil被GSK-3β磷酸化。Axil不仅与GSK-3β结合，而且与β-catenin结合，并且Axil的GSK-3β结合位点与β-catenin结合位点不同。此外，Axil增强β-catenin的GSK-3β依赖性磷酸化。这些结果表明Axil通过介导GSK-3β-依赖性β-连环蛋白磷酸化来负调节Wnt信号通路，从而抑制轴形成。
ABSTRACT Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3β (GSK-3β). This protein had 44% amino acid identity with Axin, a negative regulator of the Wnt signaling pathway.We designated this protein Axil for Axin like. Like Axin, Axil ventralized Xenopus embryos and inhibited Xwnt8-induced Xenopus axis duplication. Axil was phosphorylated by GSK-3β. Axil bound not only to GSK-3β but also to β-catenin, and the GSK-3β-binding site of Axil was distinct from the β-catenin-binding site. Furthermore, Axil enhanced GSK-3β-dependent phosphorylation of β-catenin. These results indicate that Axil negatively regulates the Wnt signaling pathway by mediating GSK-3β-dependent phosphorylation of β-catenin, thereby inhibiting axis formation.
DOI：
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发表时间：
1994-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Qiuling Wang;C. Fiol;A. DePaoli-Roach;P. Roach
通讯作者：
Qiuling Wang;C. Fiol;A. DePaoli-Roach;P. Roach
影响因子：
10.5
作者：
Yost, C;Torres, M;Moon, RT
通讯作者：
Moon, RT