Live fast, die fast principle in a single cell of fission yeast.
Live fast, die fast principle in a single cell of fission yeast.
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DOI：
10.15698/mic2017.09.591
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发表时间：
2017-08-13
期刊：
影响因子：
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通讯作者：
Nakaoka H
中科院分区：
文献类型：
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作者：
Nakaoka H
生长和死亡都是所有生物的基本宏观特性，因此细胞分裂率和死亡率是表征特定环境下细胞生理的良好参数。虽然文献中报道了各种条件下的人口增长率，但很少测量死亡率，特别是在细胞呈指数增长的有利培养条件下。在我们最近的研究中（Nakaoka和Wakamoto， 2017），我们开发了一个基于微流体的平台来跟踪多个单细胞谱系直到死亡。该系统使我们能够在可控的稳定环境中监测细胞生长和死亡，我们通过显示细胞分裂和死亡率的显著稳定性，证实了裂变酵母老极细胞系中不存在复制老化。此外，我们发现在非胁迫条件下存在生长-死亡权衡关系。约束宏观生理参数的现象学规律可以为各种环境下可能的平衡生长状态提供新的定量见解。
Growth and death are both fundamental macroscopic properties for all living matters, and thus cell division and mortality rates are good parameters for characterizing cellular physiology in a given environment. While population growth rates in various conditions have been reported in literature, death rate is rarely measured, especially in favorable culture conditions where cells grow exponentially. In our recent study (Nakaoka and Wakamoto, 2017), we developed a microfluidics-based platform to track multiple single cell lineages until death. The system enabled us to monitor both cell growth and death in controlled steady environments, and we confirmed the absence of replicative aging in fission yeast old-pole cell lineages by showing remarkable constancy both in cell division and mortality rates. Furthermore, we revealed a growth-death trade-off relation in non-stressed conditions. The phenomenological law that constrains macroscopic physiological parameters could provide a new quantitative insight into possible balanced-growth states in various environments.