Development of Vasoactive Intestinal Peptide mRNA Rhythm in the Rat Suprachiasmatic Nucleus
Development of Vasoactive Intestinal Peptide mRNA Rhythm in the Rat Suprachiasmatic Nucleus
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大鼠视交叉上核血管活性肠肽 mRNA 节律的发育
DOI：
10.1523/jneurosci.17-10-03920.1997
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Okamura
中科院分区：
文献类型：
--
作者：
Y. Ban;Y. Shigeyoshi;H. Okamura
采用原位杂交技术研究了大鼠视交叉上核（SCN）血管活性肠肽（VIP） mRNA的昼夜节律。视交叉上核是昼夜节律的主要位点。VIP神经元的表型表达发生在两个发育阶段：第一个表型表达发生在出生前的吻侧，第二个表型表达发生在出生后(P) 10至P20之间的主部位。后者与大量vip传出纤维投射到室旁下区的时间一致。在成人和P20中，光/暗（LD）条件下，SCN VIP mRNA信号表现出明显的昼夜节律，在光相有低谷，在暗相有高峰，而在恒定暗（DD）条件下，VIP mRNA没有波动。然而，在P10时，我们发现在LD条件下，SCN VIP mRNA在夜间到白天的过渡中出现峰值，在黑暗初期出现低谷，与成人节律中的夜间峰值形成鲜明对比。在DD条件下，在P10也观察到光相峰和暗相谷，与成虫期的心律失常特征形成对比。目前的研究结果表明，每日VIP节律首先在早期发育的时钟控制的吻侧SCN神经元中产生，后来由依赖光的主要VLSCN神经元调节。
Development of the daily rhythm of vasoactive intestinal peptide (VIP) mRNA in the rat suprachiasmatic nucleus (SCN), a main locus of circadian oscillation, was investigated by in situ hybridization. The phenotypic expression of VIP neurons occurred in two developmental stages in the ventrolateral portion of the SCN (VLSCN): the first was found before birth in the rostral part, and the second occurred in the main part between postnatal day (P) 10 and P20. The latter period coincided with the time that the massive VIP-efferent fibers project to the subparaventricular zone. In the adult and P20, the VIP mRNA signals of the SCN showed a clear diurnal rhythm with a trough in the light phase and a peak in the dark phase under light/dark (LD) conditions, but under constant dark (DD) conditions, no VIP mRNA fluctuations were observed. At P10, however, it was found that SCN VIP mRNA showed a peak at the transition from night to day and a trough at early dark period in LD conditions, in sharp contrast to the night peak in the adult rhythm. In DD conditions, a light-phase peak and a dark-phase trough were also observed at P10, contrasting the arrhythmic feature at adult stage. The present findings suggest that daily VIP rhythm was first generated in the early developed clock-controlled rostral SCN neurons, and later regulated by light-dependent main VLSCN neurons.
影响因子：
4.7
作者：
Huang, CS;Narahashi, T
通讯作者：
Narahashi, T