Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency
Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency
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DOI：
10.1073/pnas.95.12.7220
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发表时间：
1998-06-09
影响因子：
11.1
通讯作者：
Eide, D
中科院分区：
文献类型：
--
作者：
Grotz, N;Fox, T;Eide, D
全世界数百万人患有锌等必需金属的营养失衡。这些相同的金属，沿着镉和铅等污染物，污染了世界上许多地方的土壤。除了对人类健康构成威胁外，这些金属还会毒害植物、牲畜和野生动物。解读金属是如何被吸收、运输和作为蛋白质辅因子结合的，可能有助于解决这两个问题。例如，可食用植物可以被改造成更好的金属营养素膳食来源，其他植物物种可以被改造成从受污染的土壤中去除金属离子。我们在这里报告的第一个锌转运蛋白基因的克隆植物，ZIP 1，ZIP 2和ZIP 3基因的拟南芥。这些密切相关的基因在酵母中的表达赋予锌吸收活性。在植物中，ZIP 1和ZIP 3在根中表达以响应锌缺乏，这表明它们将锌从土壤运输到植物中。虽然ZIP 2的表达尚未被检测到，但通过基因组测序鉴定的第四个相关的拟南芥基因ZIP 4在锌限制植物的芽和根中都被诱导。因此，ZIP？可以在细胞内或植物组织之间运输锌;这些ZIP蛋白定义了一个在植物、原生动物、真菌、无脊椎动物和脊椎动物中发现的金属离子转运蛋白家族，使得现在可以解决不同生物体中金属离子积累和稳态的问题。
Millions of people worldwide suffer from nutritional imbalances of essential metals like zinc. These same metals, along with pollutants like cadmium and lead, contaminate soils at many sites around the world. In addition to posing a threat to human health, these metals can poison plants, livestock, and wildlife. Deciphering how metals are absorbed, transported, and incorporated as protein cofactors may help solve both of these problems. For example, edible plants could be engineered to serve as better dietary sources of metal nutrients, and other plant species could be tailored to remove metal ions from contaminated soils. We report here the cloning of the first zinc transporter genes from plants, the ZIP1, ZIP2, and ZIP3 genes of Arabidopsis thaliana. Expression in yeast of these closely related genes confers zinc uptake activities. In the plant, ZIP1 and ZIP3 are expressed in roots in response to zinc deficiency, suggesting that they transport zinc from the soil into the plant. Although expression of ZIP2 has not been detected, a fourth related Arabidopsis gene identified by genome sequencing, ZIP4, is induced in both shoots and roots of zinc-limited plants. Thus, ZIP? may transport zinc intracellularly or between plant tissues; These ZIP proteins define a family of metal ion transporters that are found in plants, protozoa, fungi, invertebrates, and vertebrates, making it now possible to address questions of metal ion accumulation and homeostasis in diverse organisms.