Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury: a biochemical and immunohistochemical study.
Significance of alcohol dehydrogenase (ADH) as a marker of hepatic centrilobular injury: a biochemical and immunohistochemical study.
复制标题
乙醇脱氢酶（ADH）作为肝小叶中心损伤标志物的意义：生化和免疫组织化学研究。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
J. von Wartburg
中科院分区：
文献类型：
--
作者：
D. Ito;H. Ishii;S. Kato;T. Takagi;S. Takekawa;S. Aiso;M. Tsuchiya;R. Buehler;J. von Wartburg
由于我们以前证明乙醇脱氢酶(ADH)主要分布在肝腺泡的第三区，因此我们研究了测定血清ADH活性在评估肝损伤中的作用。从溴苯、低氧和急性乙醇所致实验性肝损伤的结果推断，血清ADH活性的测定对于检测急性和早期小叶中心肝损伤是有用的。而慢性给予乙醇的大鼠在产生急性酒精负荷后，血清ADH活性略有升高，而肝脏ADH含量有下降的趋势。此外，肝脏ADH活性在肝腺泡3区有相当选择性的降低。因此，可以想象，慢性酒精对肝脏的损伤可能导致肝细胞持续释放ADH，导致血液中的酶水平相对较低的升高，而不是酶的突然释放导致血清酶活性的急剧增加。
Since we demonstrated previously that alcohol dehydrogenase (ADH) is distributed predominantly in zone 3 of the hepatic acinus, we have investigated the usefulness of determinations of serum ADH activity in the assessment of hepatic injuries. The measurement of serum ADH activity appears to be useful for the detection of acute and early centrilobular hepatic damage, as deduced from the results obtained from experimental hepatic injuries produced by bromobenzene, hypoxia and acute administration of ethanol. Whereas serum ADH activity increased slightly after the generation of an acute ethanol load in rats which were given ethanol chronically, the hepatic content of ADH tended to decrease. Moreover, a rather selective reduction of hepatic ADH activity was found in zone 3 of the hepatic acinus. Thus, it is conceivable that chronic alcoholic injury to the liver may result in persistent release of ADH from hepatocytes resulting in a relatively low elevation of the level of the enzyme in the blood, rather than a sudden release of the enzyme leading to a sharp increase in the serum enzyme activity.