SYNTHETIC PENTAPEPTIDE FROM THE B1 CHAIN OF LAMININ PROMOTES B16F10 MELANOMA CELL-MIGRATION
SYNTHETIC PENTAPEPTIDE FROM THE B1 CHAIN OF LAMININ PROMOTES B16F10 MELANOMA CELL-MIGRATION
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DOI：
10.1002/jcp.1041340216
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发表时间：
1988-02-01
影响因子：
5.6
通讯作者：
YAMADA, Y
中科院分区：
文献类型：
--
作者：
IWAMOTO, Y;GRAF, J;YAMADA, Y
层粘连蛋白是基底膜特异性糖蛋白，其促进细胞粘附、增殖、分化和肿瘤细胞迁移。从层粘连蛋白B1链的cDNA克隆推导的氨基酸序列合成的肽被测试其促进B16 F10黑素瘤细胞迁移的能力。发现能够介导上皮细胞附着于层粘连蛋白的肽CDPGYIGSR促进迁移，并且组成五肽YIGSR也是活性的，但程度较低。这九个氨基酸的肽阻断了黑色素瘤细胞迁移到层粘连蛋白，但没有迁移到纤连蛋白的影响。这些数据表明，层粘连蛋白上的细胞结合位点和迁移位点共享层粘连蛋白所特有的共同序列。
Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cells. A peptide, CDPGYIGSR, that is able to mediate epithelial cell attachment to laminin was found to promote migration, and the constituent pentapeptide YIGSR was also active but to a lesser degree. This nine-amino acid peptide blocked migration of melanoma cells to laminin but had no effect on migration to fibronectin. These data suggest that the cell-binding site and migration site on laminin share a common sequence that is unique to laminin.