A transcriptome analysis uncovers Panax notoginseng resistance to Fusarium solani induced by methyl jasmonate
A transcriptome analysis uncovers Panax notoginseng resistance to Fusarium solani induced by methyl jasmonate
复制标题
转录组分析揭示三七对茉莉酸甲酯诱导的茄病镰孢菌的抗性
DOI：
10.1007/s13258-019-00865-z
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发表时间：
2019-12-01
期刊：
影响因子：
2.1
通讯作者：
Ge, Feng
中科院分区：
文献类型：
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作者：
Liu, Diqiu;Zhao, Qin;Ge, Feng
三七是我国著名的中药材，但以茄病镰刀菌为主要病原的根腐病严重降低了三七药材的产量和质量。三七通过外源信号分子的作用而启动防御，为三七抗病性的外源调控提供理论支持。外源茉莉酸甲酯（MeJA）处理提高了三七对赤霉病的抗性。索拉尼此外，三七在F。为了揭示茉莉酸甲酯（MeJA）诱导三七疫霉菌产生抗性的机制，通过下一代测序技术研究了番茄疫霉菌对MeJA的抗性。转录组序列的从头组装产生了80，551个unigenes，并且这些unigenes中的36，771个被至少一个数据库注释。差异表达基因分析显示，大量的基因相关的萜类骨架生物合成，苯丙氨酸代谢，植物-病原体相互作用的MeJA主要上调。此外，茉莉酸（JA）生物合成相关基因和JA信号通路基因，如亚油酸13 S-脂氧合酶、丙二烯氧化物环化酶、丙二烯氧化物合成酶、TIFY、防御素和病程相关蛋白，在接种F.索拉尼基因表达分析结果表明，JA和乙烯信号通路可能协同作用，正调控三七对番茄枯萎病的防御反应。索拉尼JA信号在三七对F.研究结果为三七根腐病的环境友好型生物防治提供了理论依据。
Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials. The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng. In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng’s resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solani infection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA. The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant–pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani. JA signaling appears to play a vital role in P. notoginseng responses to F. solani infection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.