Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis
Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis
复制标题
通过酵母双杂交分析与猪瘟病毒非结构蛋白2相关的相互作用细胞蛋白
DOI：
10.1007/s11033-012-1936-x
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Lin, Zhi
中科院分区：
文献类型：
--
作者：
Kang, Kai;Guo, Kangkang;Lin, Zhi
猪瘟是由猪瘟病毒（classical swine fever virus，CSFV）引起的，CSFV对内皮细胞具有特殊的亲和力。这种发热的特点是血管损伤出血和坏死。关于血管内皮细胞和CSFV之间相互作用的信息非常少。本研究利用RNA转录本5′端转换机制构建了猪脐静脉内皮细胞（SUVEC）cDNA文库。采用酵母双杂交（Y2 H）系统筛选SUVEC中与非结构蛋白2（NS 2）相互作用的蛋白。与NCBI数据库比对发现了11个相互作用蛋白：GOPC、HNRNPH 1、DNAJA 1、ATP 6、CSDE 1、CNDP 2、FANCL、TMED 4、DNAJA 4、MOAP 1和PNMA 1。这些蛋白主要与细胞凋亡、应激反应、氧化还原或代谢有关。在基于蛋白质与NS 2构建的蛋白质相互作用网络中，较重要的蛋白质是MOAP 1、DNAJA 1、GOPC、FANCL、TMED 4和CSDE 1。Y2 H探测到的相互作用应仅被视为初步迹象。然而，在本研究中获得的SUVEC cDNA文库中的CSFV NS 2相互作用蛋白为获得更好地了解CSFV NS 2蛋白的宿主蛋白-病毒相互作用提供了有价值的信息。
Classical swine fever is caused by the classical swine fever virus (CSFV), which has a special affinity to endothelial cells. This fever is characterized by hemorrhage and necrosis of vascular injury. Very little information is available on the interaction between vascular endothelial cells and CSFV. In the current report, the cDNA library of swine umbilical vein endothelial cell (SUVEC) was constructed by the switching mechanism at 5′ end of the RNA transcript approach. The yeast two-hybrid (Y2H) system was adopted to screen non-structure 2 protein (NS2) interactive proteins in the SUVEC line. Alignment with the NCBI database revealed 11 interactive proteins: GOPC, HNRNPH1, DNAJA1, ATP6, CSDE1, CNDP2, FANCL, TMED4, DNAJA4, MOAP1, and PNMA1. These proteins were mostly related to apoptosis, stress response and oxidation reduction, or metabolism. In the protein interaction network constructed based on proteins with NS2, the more important proteins were MOAP1, DNAJA1, GOPC, FANCL, TMED4, and CSDE1. The interactions detected by the Y2H should be regarded only as preliminary indications. However, the CSFV NS2 interactive proteins in the SUVEC cDNA library obtained in the current study provides valuable information for gaining a better understanding of the host protein-virus interactions of the CSFV NS2 protein.