The role of stress proteins in responses of a montane willow leaf heetle to environmental temperature variation
The role of stress proteins in responses of a montane willow leaf heetle to environmental temperature variation
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DOI：
10.1007/s12038-007-0047-7
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发表时间：
2007-04-01
影响因子：
2.9
通讯作者：
Rank, Nathan E.
中科院分区：
文献类型：
--
作者：
Dahlhoff, Elizabeth P.;Rank, Nathan E.
热休克反应是生物体缓冲可变和不可预测的环境温度影响的关键机制。热休克蛋白（Hsps）的上调增加了暴露于自然界中的应激条件后的存活率，尽管Hsps表达的益处通常被生长和繁殖成功的代价所平衡。热休克蛋白辅助折叠的变体多肽可以防止不适合的表型的发展，因此，在热休克蛋白表达的一些差异之间的自然种群的外温动物可能是由于酶的变体（等位酶）和热休克蛋白之间的相互作用。在塞拉利昂杨柳叶甲Aeneicollis，它生活在高度可变的热栖息地在其范围的南部边缘在东部塞拉利昂内华达州，加州，等位基因频率在酶位点磷酸葡萄糖异构酶（PGI）的变化跨越气候纬度梯度。PGI等位酶的动力学特性不同，70 kDa热休克蛋白的表达不同的人群之间，沿着海拔梯度，和PGI基因型。PGI基因型之间Hsp70表达的差异对应于耐热性和繁殖成功的重要性状，如跑步速度，存活率和繁殖力的差异。因此，不同基因型之间的热休克蛋白表达差异可能允许功能上重要的遗传变异持续存在，使人口有效地应对环境变化。
The heat shock response is a critical mechanism by which organisms buffer effects of variable and unpredictable environmental temperatures. Upregulation of heat shock proteins (Hsps) increases survival after exposure to stressful conditions in nature, although benefits of Hsp expression are often balanced by costs to growth and reproductive success. Hsp-assisted folding of variant polypeptides may prevent development of unfit phenotypes; thus, some differences in Hsp expression among natural populations of ectotherms may be due to interactions between enzyme variants (allozymes) and Hsps. In the Sierra willow leaf beetle Chrysomela aeneicollis, which lives in highly variable thermal habitats at the southern edge of their range in the Eastern Sierra Nevada, California, allele frequencies at the enzyme locus phosphoglucose isomerase (PGI) vary across a climatic latitudinal gradient. PGI allozymes differ in kinetic properties, and expression of a 70 kDa Hsp differs between populations, along elevation gradients, and among PGI genotypes. Differences in Hsp70 expression among PGI genotypes correspond to differences in thermal tolerance and traits important for reproductive success, such as running speed, survival and fecundity. Thus, differential Hsp expression among genotypes may allow functionally important genetic variation to persist, allowing populations to respond effectively to environmental change.