Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression
Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression
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硫化氢通过调节抗氧化防御和衰老相关基因表达来减轻西兰花采后衰老
DOI：
10.1021/jf4047122
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发表时间：
2014-02-05
影响因子：
6.1
通讯作者：
Zhang, Hua
中科院分区：
文献类型：
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作者：
Li, Shi-Ping;Hu, Kang-Di;Zhang, Hua
越来越多的证据表明，硫化氢(H_2S)在植物体内起信号调节作用。在这里，我们证明了硫化氢以剂量依赖的方式延缓了西兰花采后的衰老。硫化氢维持了较高水平的代谢物，如类胡萝卜素、花青素和抗坏血酸，并减少了丙二醛、过氧化氢和超氧阴离子的积累。进一步的研究表明，与水对照相比，H_2S具有较高的愈创木酚过氧化物酶、抗坏血酸过氧化物酶、过氧化氢酶和谷胱甘肽还原酶活性，而较低的脂肪氧合酶、多酚氧化酶、苯丙氨酸解氨酶和蛋白酶活性。此外，青花菜叶绿素降解相关基因BoSGR、BoCLH2、BoPaO、BoRCCR以及半胱氨酸蛋白酶BoCP1和脂肪氧合酶基因BoLOX1的表达均受到抑制。H_2S对其他蔬菜和水果衰老的影响表明，H_2S具有普遍的衰老调节作用。
Accumulating evidence has shown that hydrogen sulfide (H2S) acts as a signaling regulator in plants. Here we show that H2S delays the postharvest senescence of broccoli in a dose-dependent manner. H2S maintains higher levels of metabolites, such as carotenoids, anthocyanin, and ascorbate, and reduces the accumulation of malondialdehyde, H2O2, and the superoxide anion. Further investigations showed that H2S sustained higher activities of guaiacol peroxidase, ascorbate peroxidase, catalase, and glutathione reductase and lower activities of lipoxygenase, polyphenol oxidase, phenylalanine ammonia lyase, and protease than those of water control. Moreover, the expression of the chlorophyll degradation related genes BoSGR, BoCLH2, BoPaO, BoRCCR, as well as cysteine protease BoCP1 and lipoxygenase gene BoLOX1, was down-regulated in postharvest broccoli treated with H2S. The functions of H2S on the senescence of other vegetables and fruits suggest its universal role acting as a senescence regulator.