A new chemical probe for proteomics of carbohydrate-binding proteins
A new chemical probe for proteomics of carbohydrate-binding proteins
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DOI：
10.1002/cbic.200400209
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发表时间：
2005-02-01
期刊：
影响因子：
3.2
通讯作者：
Pieters, RJ
中科院分区：
文献类型：
--
作者：
Ballell, L;Alink, KJ;Pieters, RJ
半乳糖凝集素的选择性捕获，而留下其他蛋白质不变，实现了通过激活光亲和标记，精确地定位在非共价结合的碳水化合物配体。随后通过“点击”罗丹明部分使标记的蛋白质在凝胶中可视化。
Selective capture of galectins, while leaving other proteins untouched, was achieved by activating photoaffinity labels that were precisely positioned on noncovalently bound carbohydrate ligands. The labelled proteins were visualised in-gel by “clicking-on” a rhodamine moiety afterwards.