Sporothrix brasiliensis, S-globosa, and S-mexicana, three new Sporothrix species of clinical interest
Sporothrix brasiliensis, S-globosa, and S-mexicana, three new Sporothrix species of clinical interest
复制标题
DOI：
10.1128/jcm.00808-07
复制
发表时间：
2007-10-01
影响因子：
9.4
通讯作者：
Guarro, Josep
中科院分区：
文献类型：
--
作者：
Marimon, Rita;Cano, Josep;Guarro, Josep
申克孢子丝菌是引起孢子毛病的一种真菌，孢子毛病是由这种二形真菌的创伤性植入引起的真菌感染。最近的分子研究表明，该物种构成了许多系统发育物种的复合体。由于这种物种的划分从临床角度来看可能是极其重要的，我们研究了127株分离株，其中大部分被认为是申克氏沙门氏菌，包括目前被认为是同义词的物种的现有类型菌株，以及一些形态学上接近的物种。我们使用不同的培养基、不同温度下的生长速率和大量的营养试验对所有这些分离株进行了表型表征，并比较了它们的钙调素基因序列。分子分析结果表明，白色孢子丝菌、膨胀孢子丝菌和申氏孢子丝菌是与申氏孢子丝菌明显不同的菌种。结合这些表型和遗传方法，我们提出了巴西孢子菌（Sporothrix brasiliensis）、S. globosa和S. mexicana这三个新种。识别这些物种的关键表型特征是无柄色素分生孢子的形态，在30、35和37℃下的生长，以及蔗糖、棉子糖和梨糖醇的同化。
Sporothrix schenckii is the species responsible for sporotrichosis, a fungal infection caused by the traumatic implantation of this dimorphic fungus. Recent molecular studies have demonstrated that this species constitutes a complex of numerous phylogenetic species. Since the delineation of such species could be of extreme importance from a clinical point of view, we have studied a total of 127 isolates, most of which were received as S. schenckii, including the available type strains of species currently considered synonyms, and also some close morphological species. We have phenotypically characterized all these isolates using different culture media, growth rates at different temperatures, and numerous nutritional tests and compared their calmodulin gene sequences. The molecular analysis revealed that Sporothrix albicans, S. inflata, and S. schenckii var. luriei are species that are clearly different from S. schenckii. The combination of these phenetic and genetic approaches allowed us to propose the new species Sporothrix brasiliensis, S. globosa, and S. mexicana. The key phenotypic features for recognizing these species are the morphology of the sessile pigmented conidia, growth at 30, 35, and 37 degrees C, and the assimilation of sucrose, raffinose, and ribitol.