Immune efficacy of DNA vaccines based on oprL and oprF genes of Pseudomonas aeruginosa in chickens
Immune efficacy of DNA vaccines based on oprL and oprF genes of Pseudomonas aeruginosa in chickens
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DOI：
10.3382/ps/pey307
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发表时间：
2018-12-01
期刊：
影响因子：
4.4
通讯作者：
Guo, J. Z.
中科院分区：
文献类型：
--
作者：
Gong, Q.;Ruan, M. D.;Guo, J. Z.
铜绿假单胞菌（P. aeruginosa）是一种人畜共患病原体，可感染多种动物，包括家禽。然而，由于没有商业疫苗，因此开发新的有效疫苗势在必行。本研究利用铜绿假单胞菌的oprL和oprF基因构建了2种单价DNA疫苗（pOPRL和pOPRF）、1种二价DNA疫苗（pOPRL+pOPRF）和1种融合DNA疫苗（pOPRLF）。将这些疫苗给予鸡，外膜蛋白疫苗（OMP疫苗）和灭活疫苗用作阳性对照。测定血清抗体、干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）和白细胞介素-4（IL-4）浓度，并进行淋巴细胞增殖试验。在用毒性铜绿假单胞菌攻击后，评价保护效力。免疫后，免疫鸡血清抗体、刺激指数（SI）、IL-2和IFN-γ浓度均显著高于免疫鸡。双价联合DNA疫苗组的免疫指标高于融合DNA疫苗组。而IL-4在4个DNA疫苗组间无显著性差异。pOPRL、pOPRF、pOPRLF、pOPRL+pOPRF、灭活疫苗和OMP疫苗的保护率分别为53.3%、40%、66.7%、80%、93.3%和80%。结果表明，利用铜绿假单胞菌oprL和oprF基因构建的DNA疫苗，特别是二价组合DNA疫苗，是一种较有潜力的疫苗。本研究为铜绿假单胞菌DNA疫苗的设计和应用奠定了基础。
Pseudomonas aeruginosa (P. aeruginosa) is a zoonotic pathogen that can infect a variety of animals, including poultry. However, as there is no commercial vaccine available it is imperative that new and effective vaccines are developed. In this study, 2 monovalent DNA vaccines (pOPRL and pOPRF), one divalent combination DNA vaccine (pOPRL+pOPRF) and one fusion DNA vaccine (pOPRLF) were constructed based on the oprL and oprF genes of P. aeruginosa. These vaccines were administered to chickens, an outer membrane protein vaccine (OMP vaccine) and inactivated vaccine used as positive controls. The serum antibody, interferon-gamma (IFN-gamma), interleukin-2 (IL-2) and interleukin-4 (IL-4) concentrations were determined and lymphocyte proliferation assays were performed. After challenging with virulent P. aeruginosa, protective efficacy was evaluated. Following vaccination, serum antibodies, stimulation index (SI) values, concentrations of IL-2 and IFN-gamma in chickens vaccinated with the bivalent combination DNA vaccine and fusion DNA vaccine were found to be significantly higher than in those chickens vaccinated with the 2 monovalent DNA vaccines. Moreover, the immune indexes in the bivalent combination DNA vaccine group were higher than those in the fusion DNA vaccine group. However, the concentrations of IL-4 in the 4 DNA vaccine groups were of no significant difference. The protective efficacy rate provided by pOPRL, pOPRF, pOPRLF, pOPRL+pOPRF, inactivated vaccine and OMP vaccine were 53.3%, 40%, 66.7%, 80%, 93.3%, and 80%, respectively. The results indicate that DNA vaccines constructed with the oprL and oprF genes of P. aeruginosa, particularly the divalent combination DNA vaccine, represent better potential vaccines. This study has laid a foundation for the design and application of future DNA vaccines of P. aeruginosa.