Identification of AII amacrine, displaced amacrine, and bistratified ganglion cell types in human retina with antibodies against calretinin
Identification of AII amacrine, displaced amacrine, and bistratified ganglion cell types in human retina with antibodies against calretinin
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DOI：
10.1002/cne.23821
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Gruenert, Ulrike
中科院分区：
文献类型：
--
作者：
Lee, Sammy C. S.;Weltzien, Felix;Gruenert, Ulrike
抗钙视网膜蛋白的抗体是一些但不是所有哺乳动物物种视网膜中杆状通路中间神经元（Aβ无长突细胞）的一种标记。 A？细胞在夜间（暗视）视力中发挥着至关重要的作用，并已被提议作为视网膜疾病视力光遗传学恢复的靶标。在本研究中，我们的目的是表征 A？人类视网膜中的细胞。经伦理批准获得死后人类供体眼睛，并进行钙视网膜蛋白免疫荧光处理。内核和神经节细胞层中的钙结合蛋白阳性体细胞充满了亲脂性染料 DiI。绝大多数（超过80%）的钙结合蛋白免疫反应性细胞位于内核层，甘氨酸转运蛋白1呈免疫阳性，并显示出Aβ的典型形态。无长突细胞。此外，内核层和神经节细胞层中的一小部分钙结合蛋白阳性细胞是谷氨酸脱羧酶阳性，并显示出宽视野无长突细胞（星状、半月形和刺状无长突细胞）的形态。神经节细胞层中大约一半的钙结合蛋白细胞是双层神经节细胞，类似于之前在灵长类动物中描述的小型双层神经节细胞（推测为蓝色开启/黄色关闭）和 G17 神经节细胞。我们得出的结论是，在人视网膜中，抗钙结合蛋白抗体可用于识别 A？内核层中的无长突细胞以及神经节细胞层中的宽场无长突细胞和小双层神经节细胞。 (c) 2015 年 Wiley 期刊公司。
Antibodies against calretinin are markers for one type of rod pathway interneuron (A? amacrine cell) in the retina of some but not all mammalian species. The A? cells play a crucial role in night-time (scotopic) vision and have been proposed as a target for optogenetic restoration of vision in retinal disease. In the present study we aimed to characterize the A? cells in human retina. Postmortem human donor eyes were obtained with ethical approval and processed for calretinin immunofluorescence. Calretinin-positive somas in the inner nuclear and the ganglion cell layer were filled with the lipophilic dye DiI. The large majority (over 80%) of calretinin-immunoreactive cells is located in the inner nuclear layer, is immunopositive for glycine transporter 1, and shows the typical morphology of A? amacrine cells. In addition, a small proportion of calretinin-positive cells in the inner nuclear layer and in the ganglion cell layer is glutamic acid decarboxylase-positive and shows the morphology of widefield amacrine cells (stellate, semilunar, and thorny amacrine cells). About half of the calretinin cells in the ganglion cell layer are bistratified ganglion cells resembling the small bistratified (presumed blue-ON/yellow-OFF) and the G17 ganglion cell previously described in primates. We conclude that in human retina, antibodies against calretinin can be used to identify A? amacrine cells in the inner nuclear layer as well as widefield amacrine and small bistratified ganglion cells in the ganglion cell layer. (c) 2015 Wiley Periodicals, Inc.