New insights into ice growth and melting modifications by antifreeze proteins.
New insights into ice growth and melting modifications by antifreeze proteins.
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DOI：
10.1098/rsif.2012.0388
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发表时间：
2012-12-07
期刊：
影响因子：
--
通讯作者：
Braslavsky I
中科院分区：
文献类型：
--
作者：
Bar-Dolev M;Celik Y;Wettlaufer JS;Davies PL;Braslavsky I
抗冻蛋白（AFP）在许多生物体中进化，通过控制冰晶生长使它们能够在寒冷的气候中生存。AFP与冰的特异性相互作用决定了其在农业、食品保鲜和医药等方面的潜在应用。AFP以特定AFP类型的特征方式控制冰晶的形状。中度活性的AFP导致形成细长的双锥体晶体，通常具有表面上定义的小平面，而过度活性的AFP产生更多样的晶体形状。这些不同的形态通常被认为是生长形状。在对含有不同AFP的溶液中的冰晶进行的一系列明亮的光和荧光显微镜观察中，我们表明晶体成形也发生在融化过程中。特别是，在大多数超活性AFP的溶液中观察到的特征冰形状是在融化过程中形成的。我们将这些发现与过度活跃的AFP对冰基面的亲和力有关。我们的研究结果表明基面亲和力和多动之间的关系，并显示出明显的差异，在大多数中度和多动AFP的冰成形机制。这项研究提供了与识别多动AFP相关的关键方面。
Antifreeze proteins (AFPs) evolved in many organisms, allowing them to survive in cold climates by controlling ice crystal growth. The specific interactions of AFPs with ice determine their potential applications in agriculture, food preservation and medicine. AFPs control the shapes of ice crystals in a manner characteristic of the particular AFP type. Moderately active AFPs cause the formation of elongated bipyramidal crystals, often with seemingly defined facets, while hyperactive AFPs produce more varied crystal shapes. These different morphologies are generally considered to be growth shapes. In a series of bright light and fluorescent microscopy observations of ice crystals in solutions containing different AFPs, we show that crystal shaping also occurs during melting. In particular, the characteristic ice shapes observed in solutions of most hyperactive AFPs are formed during melting. We relate these findings to the affinities of the hyperactive AFPs for the basal plane of ice. Our results demonstrate the relation between basal plane affinity and hyperactivity and show a clear difference in the ice-shaping mechanisms of most moderate and hyperactive AFPs. This study provides key aspects associated with the identification of hyperactive AFPs.
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影响因子：
3.8
作者：
Bar, Maya;Celik, Yeliz;Braslavsky, Ido
通讯作者：
Braslavsky, Ido
影响因子：
3.4
作者：
DeLuca, CI;Chao, H;Davies, PL
通讯作者：
Davies, PL
DOI：
10.1073/pnas.1100429108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Garnham, Christopher P.;Campbell, Robert L.;Davies, Peter L.
通讯作者：
Davies, Peter L.
影响因子：
8
作者：
CHAO, H;SONNICHSEN, FD;DAVIES, PL
通讯作者：
DAVIES, PL
DOI：
10.1073/pnas.89.19.8953
发表时间：
1992-10-01
影响因子：
11.1
作者：
CARPENTER, JF;HANSEN, TN
通讯作者：
HANSEN, TN