Taxus metabolomics:: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures
Taxus metabolomics:: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures
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DOI：
10.1016/s0031-9422(02)00711-2
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发表时间：
2003-03-01
期刊：
影响因子：
3.8
通讯作者：
Croteau, RB
中科院分区：
文献类型：
--
作者：
Ketchum, REB;Rithner, CD;Croteau, RB
以正戊烷作为相对非极性紫杉烷的来源，从东北红豆杉悬浮培养物中提取细胞。在该馏分中鉴定的13种紫杉烷中，8种在C-14处被氧化，2种以前没有描述过。这些紫杉烷，沿着与现有的紫杉烷标准品，被用来分析红豆杉x媒体cv.用茉莉酸甲酯诱导Hicksii细胞悬浮培养物以产生紫杉醇（Taxol（R））。在这些诱导培养物中产生的大多数紫杉烷代谢物在C-13处氧化，而不是C-14。(C)2003爱思唯尔科技有限公司版权所有。
Cells from suspension cultures of Taxus cuspidata were extracted with pentane as a source of relatively non-polar taxoids. Of the 13 taxoids identified in this fraction, eight were oxygenated at C-14 and two had not been previously described. These taxoids, along with existing taxoid standards, were employed to profile the metabolites of Taxus x media cv. Hicksii cell suspension cultures induced with methyl jasmonate to produce paclitaxel (Taxol(R)). The majority of the taxoid metabolites produced in these induced cultures were oxygenated at C-13, and not C-14. (C) 2003 Elsevier Science Ltd. All rights reserved.