Nanomanipulation of Individual DNA Molecules Covered by Single-Layered Reduced Graphene Oxide Sheets on a Solid Substrate
Nanomanipulation of Individual DNA Molecules Covered by Single-Layered Reduced Graphene Oxide Sheets on a Solid Substrate
复制标题
固体基底上单层还原氧化石墨烯片覆盖的单个 DNA 分子的纳米操作
DOI：
10.1021/acs.jpcb.7b05175
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Hu Jun
中科院分区：
文献类型：
--
作者：
Wang Ying;Shen Yue;Li Bin;Wang Shuo;Zhang Jinjin;Zhang Yi;Hu Jun
单DNA分子的纳米操纵在基础遗传学研究和临床分析中具有很大的潜力，是研究原子力显微镜(AFM)针尖操纵线性DNA分子时界面效应对物理化学过程影响的良好模型系统。在这里，我们证明了原子力显微镜纳米操纵可以在覆盖在单层还原氧化石墨烯薄膜上的DNA分子上进行。在PeakForce定量纳米力学模式下，可以使用AFM以良好的控制方式实现针对被覆盖DNA分子的纳米操纵，包括切割、推动和扫描操作。研究发现，切割被覆盖的DNA链所需的正常力比切断裸链所需的力大五倍以上。这项技术通过定制底层的人工DNA纳米结构，为构建石墨烯结构提供了一种独特的方法。
Nanomanipulation of single DNA molecules has great potential in fundamental genetic research and clinical analysis, and is a good model system for studying the interfacial effects on physiochemical processes, which occur when manipulating the linear DNA molecules with an atomic force microscope (AFM) tip. Here, we demonstrate that AFM nanomanipulation can be carried out on DNA molecules covered by a single-layered reduced graphene oxide sheet. Nanomanipulation, which includes cutting, pushing, and sweeping operations, specific to the covered DNA molecules can be achieved in a well-controlled manner using AFM in the PeakForce Quantitative Nano-Mechanics mode. It was found that the normal force required to cut covered DNA strands is over five times greater than that required for naked strands. This technique provides a distinctive method for the construction of graphene architecture by tailoring the underlying artificial DNA nanostructures.