Surface plasmon resonance imaging on a microchip for detection of DNA-modified gold nanoparticles deposited onto the surface in a non-cross-linking configuration.
Surface plasmon resonance imaging on a microchip for detection of DNA-modified gold nanoparticles deposited onto the surface in a non-cross-linking configuration.
复制标题
DOI：
10.1016/j.ab.2006.04.035
复制
发表时间：
2006-08
影响因子：
2.9
通讯作者：
Yasunobu Sato;Kae Sato;K. Hosokawa;M. Maeda
中科院分区：
文献类型：
--
作者：
Yasunobu Sato;Kae Sato;K. Hosokawa;M. Maeda
最近，我们报道了金纳米粒子（GNP）与完全匹配的双链体在其表面上选择性地沉积到聚（二甲基硅氧烷）（PDMS）在高盐浓度的微通道的墙壁上。在这项研究中，表面等离子体共振（SPR）成像技术应用于监测这一现象，以提高检测灵敏度和解释的现象。微芯片是通过将表面图案化的PDMS板和金薄膜沉积的玻璃基板结合而制成的。将探针寡核苷酸修饰的GNP与靶寡核苷酸杂交以制备完全匹配或单碱基错配的双链体。将杂交的GNP溶液与NaCl溶液在Y形微通道中混合。通过SPR成像检测GNP在金传感器表面上的沉积。在没有温度控制的情况下，在5min内可以以32nM（19fmol）的检测限区分靶标。详细的分析表明，GNP的种子层最初吸附到传感器表面上，而不管靶序列。因此，结合便携式SPR设备，所提出的方法是有希望的单核苷酸多态性的即时检测。
Recently we reported that gold nanoparticles (GNPs) with fully matched duplexes on their surfaces are selectively deposited onto walls of poly(dimethylsiloxane) (PDMS) microchannels at high salt concentrations. In this study, the surface plasmon resonance (SPR) imaging technique was applied to monitor this phenomenon for improvement of detection sensitivity and elucidation of the phenomenon. The microchip was fabricated by bonding a surface-patterned PDMS plate and a gold thin film-deposited glass substrate. Probe oligonucleotide-modified GNPs were hybridized with target oligonucleotides to make fully matched or single-base-mismatched duplexes. The hybridized GNP solution was mixed with an NaCl solution in a Y-shaped microchannel. The deposition of the GNPs onto the gold sensor surface was detected by SPR imaging. Discrimination of the targets was possible with limit of detection of 32nM (19fmol) without temperature control in 5min. Detailed analysis indicated that a seed layer of GNPs was initially adsorbed onto the sensor surface regardless of the target sequence. Therefore, in combination with a portable SPR device, the proposed method is promising for point-of-care testing of single-nucleotide polymorphsims.