A QUENCHED-FLOW TECHNIQUE FOR THE MEASUREMENT OF GLUCOSE INFLUX INTO HUMAN RED BLOOD-CELLS
A QUENCHED-FLOW TECHNIQUE FOR THE MEASUREMENT OF GLUCOSE INFLUX INTO HUMAN RED BLOOD-CELLS
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DOI：
10.1016/0003-2697(85)90131-9
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
WALMSLEY, AR
中科院分区：
文献类型：
--
作者：
LOWE, AG;WALMSLEY, AR
本文介绍了一种熄流装置，并用于测定氢氧化钠对2，4-二硝基苯乙酸乙酯的水解和D-[U-14C]葡萄糖在37℃时进入人的红细胞。C.葡萄糖进入红细胞是一个可饱和的过程，符合Michaelis-Menten动力学，其Km为6.6。+-。0.61 mm，0反式条件下最大葡萄糖进入速率为20.7+-。0.76mmol.(L细胞水)-1 S.所使用的技术只需要现成的实验室设备，而且应该很容易适用于其他快速运输过程的研究。
A quenched-flow apparatus is described and applied to measurements of the hydrolysis of 2,4-dinitrophenyl acetate by sodium hydroxide and the entry of D-[U-14C]glucose into human red blood cells at 37.degree. C. Glucose influx into red cells was a saturable process obeying Michaelis-Menten kinetics with a Km for glucose of 6.6 .+-. 0.61 mM and a maximum rate for glucose entry under 0 trans conditions of 20.7 .+-. 0.76 mmol (l cell water)-1 s-1. The technique used requires only readily available laboratory equipment and should be easily adaptable to the study of other rapid transport processes.